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PISCES: a protein sequence culling server

Open access
Wang, Guoli, Dunbrack, Roland L.·2003·Bioinformatics

Abstract

Summary: PISCES is a public server for culling sets of protein sequences from the Protein Data Bank (PDB) by sequence identity and structural quality criteria. PISCES can provide lists culled from the entire PDB or from lists of PDB entries or chains provided by the user. The sequence identities are obtained from PSI-BLAST alignments with position-specific substitution matrices derived from the non-redundant protein sequence database. PISCES therefore provides better lists than servers that use BLAST, which is unable to identify many relationships below 40% sequence identity and often overestimates sequence identity by aligning only well-conserved fragments. PDB sequences are updated weekly. PISCES can also cull non-PDB sequences provided by the user as a list of GenBank identifiers, a FASTA format file, or BLAST/PSI-BLAST output. Availability: The server is located at http://www.fccc.edu/research/labs/dunbrack/pisces Contact: rl_dunbrack@fccc.edu * To whom correspondence should be addressed.

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Metadata

Authors
Wang, G., & Dunbrack, R. L.
Venue
Bioinformatics
Published
2003-08-12
Volume
19
Issue
12
Pages
1589-1591
ISSN
  • 1367-4803 (ISSN-L)
  • 1367-4811
OpenAlex
W2153153865
Cited by
1,662
References
2
FWCI
38.15
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: PISCES: a protein sequence culling server. https://app.cochat.ai/papers/10.1093/bioinformatics/btg224
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Wang, G., & Dunbrack, R. L. (2003). PISCES: a protein sequence culling server. Bioinformatics. https://doi.org/10.1093/bioinformatics/btg224

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