Munc13-2 Differentially Affects Hippocampal Synaptic Transmission and Plasticity
Abstract
The short-term dynamics of synaptic communication between neurons provides neural networks with specific frequency-filter characteristics for information transfer. The direction of short-term synaptic plasticity, that is, facilitation versus depression, is highly dependent on and inversely correlated to the basal release probability of a synapse. Amongst the processes implicated in shaping the release probability, proteins that regulate the docking and priming of synaptic vesicles at the active zone are of special importance. Here, we found that a member of the Munc13 protein family of priming proteins, namely Munc13-2, is essential for normal release probability at hippocampal mossy fiber synapses. Paired pulse and frequency facilitation were strongly increased, whereas mossy fiber long-term potentiation was unaffected in the absence of Munc13-2. In contrast, transmission at 3 other types of hippocampal synapses, Schaffer-collateral, associational-commissural, as well as inhibitory synapses onto CA3 pyramidal neurons was unaffected by the loss of Munc13-2.
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bMunc13-2 is the second-most abundant isoform expressed at mossy fiber bouton synapses.
“After Munc13-1, the second-most abundant isoform expressed at MFB synapses is bMunc13-2 [40,42,77].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [77]
Cites
Distinct short-term plasticity at two excitatory synapses in the hippocampus
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Differential expression of two novel Munc13 proteins in rat brain
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Self-citationQuantal transmission at mossy fibre targets in the CA3 region of the rat hippocampus
Lawrence, J. J., Grinspan, Z. M., & McBain, C. J. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2003.049551 · Cited by 125 · see citing works
Kim, J., & Tsien, R. W. (2008) · Neuron · doi:10.1016/j.neuron.2008.05.009 · Cited by 124 · see citing works
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Rebola, N., Reva, M., Kirizs, T., Szoboszlay, M., Lőrincz, A., Moneron, G., Nusser, Z., & DiGregorio, D. A. (2019) · Neuron · doi:10.1016/j.neuron.2019.08.014 · Cited by 136 · see citing works
Chen, Z., Cooper, B., Kalla, S., Varoqueaux, F., & Young, S. M. (2013) · The Journal of Neuroscience · doi:10.1523/jneurosci.5128-12.2013 · Cited by 102 · see citing works
Self-citationKim, O., Okamoto, Y., Kaufmann, W. A., Brose, N., Shigemoto, R., & Jonas, P. (2024) · PLOS Biology · doi:10.1371/journal.pbio.3002879 · Cited by 15 · see citing works
“The Munc13-2 antibody was targeted against the brain-specific splice variant bMunc13-2, which is more abundant than the ubiquitous splice variant ubMunc13-2 in the hippocampus [77,96].”
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- Authors
- Breustedt, J., Gundlfinger, A., Varoqueaux, F., Reim, K., Brose, N., & Schmitz, D.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- Bernstein Center for Computational Neuroscience Berlin (DE)
- Max Planck Institute of Experimental Medicine (DE)
- Venue
- Cerebral Cortex
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-08-21
- Volume
- 20
- Issue
- 5
- Pages
- 1109-1120
- ISSN
- 1047-3211 (ISSN-L)
- 1460-2199
- OpenAlex
- W2140648518
- Cited by
- 51
- References
- 4
- Citations by year
- 2026: 4 · 2024: 4 · 2023: 2 · 2022: 5 · 2021: 1 · 2020: 3 · 2019: 5 · 2018: 5 · 2017: 6 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 5 · 2012: 1
- FWCI
- 0.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cerebral Cortex (publishedVersion)
- PubMed (publishedVersion)
- MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- synaptic vesicle priming, release probability, short-term plasticity, paired-pulse facilitation, frequency facilitation, long-term potentiation, synaptic vesicle docking, active zone, Schaffer collateral synapses, inhibitory synapses, CA3 pyramidal cells, synaptic transmission
- MeSH terms
- Animals, Animals, Newborn, Calcium, Cyclopropanes, Dipeptides, Electric Stimulation, Glycine, Hippocampus, Nerve Tissue Proteins, Neuronal Plasticity, Pyridazines, Quinoxalines, Synapses, Long-Term Potentiation, Mice, Knockout, Patch-Clamp Techniques, Excitatory Amino Acid Antagonists, GABA Antagonists, Mossy Fibers, Hippocampal, Excitatory Postsynaptic Potentials, Intracellular Signaling Peptides and Proteins, Mice, In Vitro Techniques
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