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Preparation and Properties of a Cholesterol Oxidase from Nocardia sp. and Its Application to the Enzymatic Assay of Total Cholesterol in Serum
Abstract
I describe the characterization, extraction, and purification of a cholesterol:oxygen oxidoreductase (EC 1.1.3.6) from Nocardia sp. This enzyme catalyzes oxidation of cholesterol to Δ4-choIestenone, with production of hydrogen peroxide. It is very stable, active over a wide pH range, and has a Km of 1.4 x 10-5 mol/ liter. It is highly specific for Δ4- or Δ5-3β-hydroxycholestanes, and may be applied to the assay of serum total cholesterol. In the procedure presented here, hydrogen peroxide is measured by reaction with quadrivalent titanium and xylenol orange. This constitutes a one-enzyme assay with stable reagents, which does not require protein precipitation and is not subject to interference from hemoglobin or bilirubin.
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Cited by
Reproducibility and validity of dietary patterns assessed with a food-frequency questionnaire
Hu, F. B., Rimm, E., Smith-Warner, S. A., Feskanich, D., Stampfer, M. J., Ascherio, A., Sampson, L., & Willett, W. C. (1999) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/69.2.243 · Cited by 1,165
Enzymatische Bestimmung des Gesamt-Cholesterins im Serum
Röschlau, P., Bernt, E., & Gruber, W. (1974) · Clinical Chemistry and Laboratory Medicine · doi:10.1515/cclm.1974.12.9.403 · Cited by 733
Enzymatic methods for quantification of lipoprotein lipids
Warnick, G. R. (1986) · Methods in Enzymology · doi:10.1016/0076-6879(86)29064-3 · Cited by 393
Yoshikawa, T., Naito, Y., Kishi, A., Tomii, T., Kaneko, T., Iinuma, S., Ichikawa, H., Yasuda, M., Takahashi, S., & Kondo, M. (1993) · Gut · doi:10.1136/gut.34.6.732 · Cited by 339
Chiba, H., Uehara, M., Wu, J., Wang, X., Masuyama, R., Suzuki, K., Kanazawa, K., & Ishimi, Y. (2003) · The Journal of Nutrition · doi:10.1093/jn/133.6.1892 · Cited by 273
Metadata
- Authors
- Richmond, W.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1973-12-01
- Volume
- 19
- Issue
- 12
- Pages
- 1350-1356
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1951207131
- Cited by
- 1,587
- References
- 0
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- Richmond, W. (1973). Preparation and Properties of a Cholesterol Oxidase from Nocardia sp. and Its Application to the Enzymatic Assay of Total Cholesterol in Serum. Clinical Chemistry. https://doi.org/10.1093/clinchem/19.12.1350
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