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Cholesterol in Serum and Lipoprotein Fractions

Anderson, Joseph T, Keys, Ancel·1956·Clinical Chemistry

Abstract

1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.

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Metadata

Authors
Anderson, J. T., & Keys, A.
Venue
Clinical Chemistry
Published
1956-06-01
Volume
2
Issue
3
Pages
145-159
ISSN
  • 0009-9147 (ISSN-L)
  • 1530-8561
OpenAlex
W1938298886
Cited by
298
References
0
FWCI
24.63
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Cholesterol in Serum and Lipoprotein Fractions. https://app.cochat.ai/papers/10.1093/clinchem/2.3.145
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Anderson, J. T., & Keys, A. (1956). Cholesterol in Serum and Lipoprotein Fractions. Clinical Chemistry. https://doi.org/10.1093/clinchem/2.3.145

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.