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Cholesterol in Serum and Lipoprotein Fractions
Abstract
1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.
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Citations
Cited by
The seven countries study: 2,289 deaths in 15 years
Keys, A., Menotti, A., Aravanis, C., Blackburn, H., Djordevič, B. S., Buzina, R., Dontas, A. S., Fidanza, F., Karvonen, M. J., Kimura, N., et al. (1984) · Preventive Medicine · doi:10.1016/0091-7435(84)90047-1 · Cited by 553
Self-citationSerum cholesterol response to changes in the diet
Keys, A., Anderson, J. T., & Grande, F. (1965) · Metabolism · doi:10.1016/0026-0495(65)90003-x · Cited by 484
Self-citationAngina pectoris among 10,000 men
Medalie, J. H., & Goldbourt, U. (1976) · The American Journal of Medicine · doi:10.1016/0002-9343(76)90921-9 · Cited by 278
Pekkanen, J., Nissinen, A., Marti, B., Tuomilehto, J., Punsar, S., & Karvonen, M. (1987) · The Lancet · doi:10.1016/s0140-6736(87)92218-5 · Cited by 182
Serum cholesterol and relative body weight of coronary patients in different populations
KEYS, A., & FIDANZA, F. (1960) · Circulation · doi:10.1161/01.cir.22.6.1091 · Cited by 74
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Metadata
- Authors
- Anderson, J. T., & Keys, A.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1956-06-01
- Volume
- 2
- Issue
- 3
- Pages
- 145-159
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1938298886
- Cited by
- 298
- References
- 0
- FWCI
- 24.63
- Type
- journal article
- Language
- en
- Open access
- closed
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- Anderson, J. T., & Keys, A. (1956). Cholesterol in Serum and Lipoprotein Fractions. Clinical Chemistry. https://doi.org/10.1093/clinchem/2.3.145
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.