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Single-Antibody Technique for Radioimmunoassay of Cortisol in Unextracted Serum or Plasma
Abstract
A radioimmunoassay procedure for cortisol is described that requires only 5 µl of serum or plasma. Native cortisol-binding proteins are denatured by heating 5 µl of sample in 0.2 ml of glutamate buffer in a boiling water bath for 15 min. After a 2-h incubation of heat-treated serum or plasma with [H3]cortisol and cortisol antibody, "free" and "bound" cortisol are separated by use of hemoglobin-coated charcoal. The method is easy and sensitive, and results compare favorably with those of a well-accepted acid-fluorescence method. In addition, we show that serum and heparinized plasma yield the same results by this method, and therefore either can be assayed by this procedure.
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Citations
Cited by
Measuring stress in wildlife: techniques for quantifying glucocorticoids
Sheriff, M. J., Dantzer, B., Delehanty, B., Palme, R., & Boonstra, R. (2011) · Oecologia · doi:10.1007/s00442-011-1943-y · Cited by 919
Nibbling versus gorging: metabolic advantages of increased meal frequency
Jenkins, D. J. A., Wolever, T. M. S., Vuksan, V., Brighenti, F., Cunnane, S. C., Rao, A. V., Jenkins, A. L., Buckley, G., Patten, R., Singer, W., et al. (1989) · New England Journal of Medicine · doi:10.1056/nejm198910053211403 · Cited by 467
Glucose metabolism in severely burned patients
Wolfe, R. R., Durkot, M. J., Allsop, J. R., & Burke, J. F. (1979) · Metabolism · doi:10.1016/0026-0495(79)90007-6 · Cited by 335
LAUE, L., LOTZE, M. T., CHROUSOS, G. P., BARNES, K., LORIAUX, D. L., & FLEISHER, T. A. (1990) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-71-6-1474 · Cited by 77
Circadian and circannual rhythms of hormonal variables in elderly men and women
Nicolau, G. Y., Lakatua, D., Sackett-Lundeen, L., & Haus, E. (1984) · Chronobiology International · doi:10.3109/07420528409063911 · Cited by 74
Metadata
- Authors
- Foster, L. B., & Dunn, R. T.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1974-03-01
- Volume
- 20
- Issue
- 3
- Pages
- 365-368
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2137514968
- Cited by
- 319
- References
- 0
- FWCI
- 9.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Foster, L. B., & Dunn, R. T. (1974). Single-Antibody Technique for Radioimmunoassay of Cortisol in Unextracted Serum or Plasma. Clinical Chemistry. https://doi.org/10.1093/clinchem/20.3.365
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
