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Cholesterol in high-density lipoprotein: use of Mg2+/dextran sulfate in its enzymic measurement.
Abstract
We describe a method for measuring high-density lipoprotein cholesterol. MgCl2 and dextran sulfate are used to precipitate all low-density and very-low-density lipoproteins. The supernate contains only high-density lipoproteins, the cholesterol concentration of which is estimated by an enzymic method, with a discrete analyzer (Abbott Bichromatic Analyzer). Concentration and instrument response are linearly related to 50 mg/liter. The precision of the method is excellent in the range of clinical interest (100 to 1000 mg of cholesterol per liter). The precision and efficiency of the precipitation are shown at various concentrations of high-density lipoprotein cholesterol. The method was compared to that of two laboratories in the Cooperative Lipoprotein Phenotyping Study group by testing a number of split samples, and agreement was good.
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Cited by
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Barnard, N. D., Cohen, J., Jenkins, D. J. A., Turner-McGrievy, G., Gloede, L., Jaster, B., Seidl, K., Green, A. A., & Talpers, S. (2006) · Diabetes Care · doi:10.2337/dc06-0606 · Cited by 468
Ginsberg, H. N., Le, N. A., Goldberg, I. J., Gibson, J. C., Rubinstein, A., Wang-Iverson, P., Norum, R., & Brown, W. V. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112713 · Cited by 424
Changes in plasma lipids and lipoproteins during isotretinoin therapy for acne.
Bershad, S., Rubinstein, A., Paterniti, J. R., Le, N.-A., Poliak, S. C., Heller, B., Ginsberg, H. N., Fleischmajer, R., & Brown, W. V. (1985) · New England Journal of Medicine · doi:10.1056/nejm198510173131604 · Cited by 217
Nicholson, A. S., Sklar, M., Barnard, N. D., Gore, S., Sullivan, R., & Browning, S. (1999) · Preventive Medicine · doi:10.1006/pmed.1999.0529 · Cited by 171
Cigarette smoking is associated with elevated adrenal androgen response to adrenocorticotropin
Hautanen, A., Mänttäri, M., Kupari, M., Sarna, S., Manninen, V., Heikki Frick, M., & Adlercreutz, H. (1993) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(93)90300-l · Cited by 44
Metadata
- Authors
- Finley, P. R., Schifman, R. B., Williams, R. J., & Lichti, D. A.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1978-06-01
- Volume
- 24
- Issue
- 6
- Pages
- 931-933
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2161305604
- Cited by
- 418
- References
- 0
- FWCI
- 13.05
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
