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Immunoturbidimetric assays of apolipoproteins A, AI, AII, and B in serum.
Abstract
In these immunoturbidimetric assays developed for apolipoproteins A, AI, AII, and B we used the Cobas-Bio centrifugal analyzer and commercially available antisera and calibrators. Within-day and within-run precision ranged from 1.0 to 5.8% for the four assays. The addition of bilirubin, up to 150 mg/L, or hemoglobin, 50 g/L, to sera did not interfere with the measurement of these apolipoproteins. Comparison of results of the immunoturbidimetric assays with those of radial immunodiffusion assays yielded slopes of 1.002, 0.822, and 0.957 and intercepts of -0.02, 0.47, and 0.03 g/L for apolipoproteins B, A, and AI, respectively. Reference intervals for the four apolipoproteins were determined by using sera from 193 apparently healthy individuals.
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Citations
Cited by
Ridker, P. M., Rifai, N., Cook, N. R., Bradwin, G., & Buring, J. E. (2005) · JAMA · doi:10.1001/jama.294.3.326 · Cited by 744
Self-citationAviram, M., Rosenblat, M., Gaitini, D., Nitecki, S., Hoffman, A., Dornfeld, L., Volkova, N., Presser, D., Attias, J., Liker, H., et al. (2004) · Clinical Nutrition · doi:10.1016/j.clnu.2003.10.002 · Cited by 622
Effects of Tamoxifen on Cardiovascular Risk Factors in Postmenopausal Women
Love, R. R., Wiebe, D. A., Newcomb, P. A., Cameron, L., Leventhal, H., Jordan, V. C., Feyzi, J., & DeMets, D. L. (1991) · Annals of Internal Medicine · doi:10.7326/0003-4819-115-11-860 · Cited by 374
Chiu, S., Bergeron, N., Williams, P. T., Bray, G. A., Sutherland, B., & Krauss, R. M. (2016) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.115.123281 · Cited by 328
Chan, P. T., Fong, W. P., Cheung, Y. L., Huang, Y., Ho, W. K. K., & Chen, Z.-Y. (1999) · The Journal of Nutrition · doi:10.1093/jn/129.6.1094 · Cited by 272
Metadata
- Authors
- Rifai, N., & King, M. E.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1986-06-01
- Volume
- 32
- Issue
- 6
- Pages
- 957-961
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2226125068
- Cited by
- 187
- References
- 0
- FWCI
- 9.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rifai, N., & King, M. E. (1986). Immunoturbidimetric assays of apolipoproteins A, AI, AII, and B in serum. Clinical Chemistry. https://doi.org/10.1093/clinchem/32.6.957
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