Frequent contamination of Taq polymerase with DNA
Integrity signals
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The narrowed specificity of the FilmArray ME Panel for E. coli was a necessary design consideration to prevent detection of contaminating E. coli nucleic acid, which is common in PCR reagents.
“The narrowed specificity of the FilmArray ME Panel for this organism was a necessary design consideration in order to prevent detection of contaminating E. coli nucleic acid, which is common in PCR reagents (e.g., DNA polymerase and reverse transcriptase) (46, 47).”
From Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens (2016) · cites this paper as [46]
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Leber, A. L., Everhart, K., Balada-Llasat, J.-M., Cullison, J., Daly, J., Holt, S., Lephart, P., Salimnia, H., Schreckenberger, P. C., DesJarlais, S., et al. (2016) · Journal of Clinical Microbiology · doi:10.1128/jcm.00730-16 · Cited by 609 · see citing works
Schuurman, T., de Boer, R. F., Kooistra-Smid, A. M. D., & van Zwet, A. A. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.2.734-740.2004 · Cited by 234 · see citing works
Lee, S. H., Chen, S.-Y., Chien, J.-Y., Lee, T.-F., Chen, J.-M., & Hsueh, P.-R. (2019) · Journal of Microbiology, Immunology and Infection · doi:10.1016/j.jmii.2019.04.005 · Cited by 54 · see citing works
- Authors
- Böttger, E. C.
- Corresponding author
- Böttger, E C
- ORCID
- Böttger, E C
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1990-06-01
- Volume
- 36
- Issue
- 6
- Pages
- 1258-1259
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1484113940
- Cited by
- 117
- References
- 0
- Citations by year
- 2026: 1 · 2024: 1 · 2021: 7 · 2019: 4 · 2018: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 5 · 2012: 5
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Keywords
- Taq DNA polymerase, DNA contamination, polymerase activity, PCR contamination, DNA amplification
- MeSH terms
- DNA-Directed DNA Polymerase, DNA, Bacterial, Drug Contamination, False Positive Reactions, Gene Amplification, Polymerase Chain Reaction, Taq Polymerase
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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