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Simultaneous determination of 6-thioguanine and methyl 6-mercaptopurine nucleotides of azathioprine in red blood cells by HPLC
Open accessAbstract
6-thioguanine (6-TGN) and methyl 6-mercaptopurine nucleotides (Me6-MPNs) are the two major metabolites found in erythrocytes after administration of azathioprine. In an attempt to understand the role of these metabolites in the pharmacologic and toxic activity of thiopurines, we have developed a HPLC method for the simultaneous determination of 6-TGNs and Me6-MPNs in erythrocytes. A simple and rapid treatment procedure based on deproteinization by perchloric acid with dithiothreitol is described. The nucleotides were hydrolyzed to their own bases by heating the sample for 45 min at 100 °C. During acid hydrolysis Me6-MP was converted into a compound analyzed on a Purospher RP18-e column with 0.02 mol/L dihydrogenophosphate buffer–methanol as eluents. With this procedure, mean recoveries of 73.1% and 84.0% for 6-TGN and Me6-MPN derivatives, respectively, were found
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Ruemmele, F. M., Veres, G., Kolho, K. L., Griffiths, A., Levine, A., Escher, J. C., Amil Dias, J., Barabino, A., Braegger, C. P., Bronsky, J., et al. (2014) · Journal of Crohn s and Colitis · doi:10.1016/j.crohns.2014.04.005 · Cited by 1,083
Karimi-Maleh, H., Shojaei, A. F., Tabatabaeian, K., Karimi, F., Shakeri, S., & Moradi, R. (2016) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2016.07.086 · Cited by 293
Metadata
- Authors
- Dervieux, T., & Boulieu, R.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-03-01
- Volume
- 44
- Issue
- 3
- Pages
- 551-555
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2158585848
- Cited by
- 215
- References
- 0
- FWCI
- 3.04
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Simultaneous determination of 6-thioguanine and methyl 6-mercaptopurine nucleotides of azathioprine in red blood cells by HPLC. https://app.cochat.ai/papers/10.1093/clinchem/44.3.551
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- Dervieux, T., & Boulieu, R. (1998). Simultaneous determination of 6-thioguanine and methyl 6-mercaptopurine nucleotides of azathioprine in red blood cells by HPLC. Clinical Chemistry. https://doi.org/10.1093/clinchem/44.3.551
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