The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Microparticles: major transport vehicles for distinct microRNAs in circulation
Open accessAbstract
AIMS: Circulating microRNAs (miRNAs) have attracted major interest as biomarkers for cardiovascular diseases. Since RNases are abundant in circulating blood, there needs to be a mechanism protecting miRNAs from degradation. We hypothesized that microparticles (MP) represent protective transport vehicles for miRNAs and that these are specifically packaged by their maternal cells. METHODS AND RESULTS: Conventional plasma preparations, such as the ones used for biomarker detection, are shown to contain substantial numbers of platelet-, leucocyte-, and endothelial cell-derived MP. To analyse the widest spectrum of miRNAs, Next Generation Sequencing was used to assess miRNA profiles of MP and their corresponding stimulated and non-stimulated cells of origin. THP-1 (monocytic origin) and human umbilical vein endothelial cell (HUVEC) MP were used for representing circulating MP at a high purity. miRNA profiles of MP differed significantly from those of stimulated and non-stimulated maternal THP-1 cells and HUVECs, respectively. Quantitative reverse transcription-polymerase chain reaction of miRNAs which have been associated with cardiovascular diseases also demonstrated significant differences in miRNA profiles between platelets and their MP. Notably, the main fraction of miRNA in plasma was localized in MP. Furthermore, miRNA profiles of MP differed significantly between patients with stable and unstable coronary artery disease. CONCLUSION: Circulating MP represent transport vehicles for large numbers of specific miRNAs, which have been associated with cardiovascular diseases. miRNA profiles of MP are significantly different from their maternal cells, indicating an active mechanism of selective 'packaging' from cells into MP. These findings describe an interesting mechanism for transferring gene-regulatory function from MP-releasing cells to target cells via MP circulating in blood.
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Mogilyansky, E., & Rigoutsos, I. (2013) · Cell Death & Differentiation · doi:10.1038/cdd.2013.125 · Cited by 889
Apoptotic Cell-Derived Extracellular Vesicles: More Than Just Debris
Caruso, S., & Poon, I. K. H. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01486 · Cited by 541
Methodological challenges in utilizing mi RNA s as circulating biomarkers
Moldovan, L., Batte, K. E., Trgovcich, J., Wisler, J., Marsh, C. B., & Piper, M. (2014) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.12236 · Cited by 436
Jansen, F., Yang, X., Hoelscher, M., Cattelan, A., Schmitz, T., Proebsting, S., Wenzel, D., Vosen, S., Franklin, B. S., Fleischmann, B. K., et al. (2013) · Circulation · doi:10.1161/circulationaha.113.001720 · Cited by 430
HDL-transferred microRNA-223 regulates ICAM-1 expression in endothelial cells
Tabet, F., Vickers, K. C., Cuesta Torres, L. F., Wiese, C. B., Shoucri, B. M., Lambert, G., Catherinet, C., Prado-Lourenco, L., Levin, M. G., Thacker, S., et al. (2014) · Nature Communications · doi:10.1038/ncomms4292 · Cited by 428
Metadata
- Authors
- Diehl, P., Fricke, A., Sander, L., Stamm, J., Bassler, N., Htun, N., Ziemann, M., Helbing, T., El-Osta, A., Jowett, J. B. M., & Peter, K.
- Venue
- Cardiovascular Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-01-18
- Volume
- 93
- Issue
- 4
- Pages
- 633-644
- ISSN
- 0008-6363 (ISSN-L)
- OpenAlex
- W2126601545
- Cited by
- 470
- References
- 48
- FWCI
- 17.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Diehl, P., Fricke, A., Sander, L., Stamm, J., Bassler, N., Htun, N., Ziemann, M., Helbing, T., El-Osta, A., Jowett, J. B. M., & Peter, K. (2012). Microparticles: major transport vehicles for distinct microRNAs in circulation. Cardiovascular Research. https://doi.org/10.1093/cvr/cvs007
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