The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Streptococcal heme binding protein (Shp) promotes virulence and contributes to the pathogenesis of group A Streptococcus infection
Open accessAbstract
Streptococcal heme binding protein (Shp) is involved in the process of heme acquisition in group A Streptococcus (GAS). However, no research thus far has examined the contribution of Shp to the virulence of GAS. To this end, we generated an isogenic strain lacking the shp gene (Δshp) and its complemented strain (Δshp-c) using the parent strain MGAS5005 (WT). Deletion of shp increased survival rates and neutrophil recruitment and reduced skin lesion sizes and GAS loads in the blood and the liver, lung, kidney and spleen in subcutaneous infections of mice. These results indicate that Shp significantly contributes to the skin and systemic invasion of GAS. The growth of the Δshp mutant was significantly slower than MGAS5005 and Δshp-c than in non-immune human blood and in incubation with isolated rat neutrophils. Microarray transcriptional analyses found no alteration in expression of virulence genes, indicating that the phenotype of the Δshp mutant was directly linked to the lack of Shp. The findings indicate that Shp significantly contributes to GAS skin invasion, systemic infection and virulence and that these contributions of Shp are mediated by the effects of Shp on systemic GAS growth and neutrophil responses.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“Our recent study (Zhang et al., 2017) found that Shp significantly contributes to GAS skin invasion, systemic infection, and virulence.”
From The Heme Transporter HtsABC of Group A Streptococcus Contributes to Virulence and Innate Immune Evasion in Murine Skin Infections (2018) · cites this paper as Zhang et al., 2017
The cited source says what the claim attributes to it.
In the citing paper
“Our laboratory recently reported that another heme uptake protein, Shp, is important for GAS virulence (Zhang et al., 2017).”
From The Heme Transporter HtsABC of Group A Streptococcus Contributes to Virulence and Innate Immune Evasion in Murine Skin Infections (2018) · cites this paper as Zhang et al., 2017
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Iron in infection and immunity
Cassat, J. E., & Skaar, E. P. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.04.010 · Cited by 1,214
Disease Manifestations and Pathogenic Mechanisms of Group A Streptococcus
Walker, M. J., Barnett, T. C., McArthur, J. D., Cole, J. N., Gillen, C. M., Henningham, A., Sriprakash, K. S., Sanderson-Smith, M. L., & Nizet, V. (2014) · Clinical Microbiology Reviews · doi:10.1128/cmr.00101-13 · Cited by 908
Streptococcus pyogenes: Basic Biology to Clinical Manifestations
Ferretti, J. J., Dl, S., & Va, F. (2016) · Cited by 565
Recent developments in understanding the iron acquisition strategies of gram positive pathogens
Sheldon, J. R., & Heinrichs, D. E. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv009 · Cited by 272
Effect of SpeB and EndoS from Streptococcus pyogenes on Human Immunoglobulins
Collin, M., & Olsén, A. (2001) · Infection and Immunity · doi:10.1128/iai.69.11.7187-7189.2001 · Cited by 205
Cole, J. N., Pence, M. A., von Köckritz‐Blickwede, M., Hollands, A., Gallo, R. L., Walker, M. J., & Nizet, V. (2010) · mBio · doi:10.1128/mbio.00191-10 · Cited by 134
Heme and virulence: how bacterial pathogens regulate, transport and utilize heme
Wilks, A., & Burkhard, K. A. (2007) · Natural Product Reports · doi:10.1039/b604193k · Cited by 130
Fernie-King, B. A., Seilly, D. J., Willers, C., Würzner, R., Davies, A., & Lachmann, P. J. (2001) · Immunology · doi:10.1046/j.1365-2567.2001.01249.x · Cited by 121
Lei, B., Smoot, L. M., Menning, H. M., Voyich, J. M., Kala, S. V., Deleo, F. R., Reid, S. D., & Musser, J. M. (2002) · Infection and Immunity · doi:10.1128/iai.70.8.4494-4500.2002 · Cited by 113
Uchiyama, S., Döhrmann, S., Timmer, A. M., Dixit, N., Ghochani, M., Bhandari, T., Timmer, J. C., Sprague, K., Bubeck-Wardenburg, J., Simon, S. I., et al. (2015) · Frontiers in Immunology · doi:10.3389/fimmu.2015.00581 · Cited by 98
Research works printed but not linked: 1
- Pancholi, 2016, Streptococcus pyogenes Metabolism, 13
Cited by
Sorted by citations
Song, Y., Zhang, X., Cai, M., Lv, C., Zhao, Y., Wei, D., & Zhu, H. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01105 · Cited by 14
“Our recent study (Zhang et al., 2017) found that Shp significantly contributes to GAS skin invasion, systemic infection, and virulence.”
BackgroundSelf-citation
Metadata
- Authors
- Zhang, X., Lu, C., Zhang, F., Song, Y., Cai, M., & Zhu, H.
- Affiliations
- Harbin Medical University (CN)
- Corresponding author
- Zhang, Xiaolan
- Lu, Chunmei
- Zhu, Hui
- Venue
- Pathogens and Disease
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-07-21
- Volume
- 75
- Issue
- 7
- ISSN
- 2049-632X (ISSN-L)
- OpenAlex
- W2737343939
- Cited by
- 8
- References
- 24
- Citations by year
- 2024: 2 · 2020: 4 · 2018: 2
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Pathogens and Disease (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chinese National Natural Science Foundation (81571957)
- Youth research and innovation foundation of Harbin Medical University (2017JCZX44, 2016JCZX35)
- Bureau of Harbin city science and technology fundation (2013RFLXJ021)
- China Postdoctoral Science Foundation (2014M551267)
- Heilongjiang Provincial Postdoctoral Science Foundation (LBH-Z13142)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Streptococcal Infections and Treatments
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and Maternal Infections
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- SHP, Streptococcus pyogenes, virulence factors, heme acquisition, neutrophil recruitment, dermal invasion, systemic infection, subcutaneous infection, mouse model
- MeSH terms
- Heme-Binding Proteins, Animals, Bacterial Proteins, Carrier Proteins, Genetic Complementation Test, Hemeproteins, Kidney, Liver, Lung, Neutrophils, Skin, Spleen, Streptococcal Infections, Streptococcus pyogenes, Virulence, Gene Expression, Gene Expression Regulation, Bacterial, Survival Analysis, Gene Deletion, Neutrophil Infiltration, Microarray Analysis, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
