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The Alcohol Dehydrogenase Gene Is Nested in the outspread Locus of Drosophila melanogaster
Open accessAbstract
This report describes the structure and expression of the outspread (osp) gene of Drosophila melanogaster. Previous work showed that chromosomal breakpoints associated with mutations of the osp locus map to both sides of the alcohol dehydrogenase gene (Adh), suggesting that Adh and the adjacent gene Adh' are nested in osp. We extended a chromosomal walk and mapped additional osp mutations to define the maximum molecular limit of osp as 119 kb. We identified a 6-kb transcript that hybridizes to osp region DNA and is altered or absent in osp mutants. Accumulation of this RNA peaks during embryonic and pupal periods. The osp cDNAs comprise two distinct classes based on alternative splicing patterns. The 5′ end of the longest cDNA was extended by PCR amplification. When hybridized to the osp walk, the 5′ extension verifies that Adh and Adh' are nested in osp and shows that osp has a transcription unit of ≥74 kb. In situ hybridization shows that osp is expressed both maternally and zygotically. In the ovary, osp is transcribed in nurse cells and localized in the oocyte. In embryos, expression is most abundant in the developing visceral and somatic musculature.
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“Finally, CG3479 is the gene mutated in the Drosophila outspread (osp) wing morphology allele, and is expressed in muscle, with one of its 2 mammalian orthologs (MPRIP) being been found to regulate actinomyosin filaments [84,85].”
From Functional unknomics: Systematic screening of conserved genes of unknown function (2023) · cites this paper as [84]
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- ASHBURNER, M., and F. LEMEUNIER, 1976 Relationships within the melanogaster species subgroup of the genus Drosophila (Sopho- phora). I. Inversion polymorphisms in Drosophila melanogasterand Dmsophilu simulum. Proc. R. SOC. Lond. B Biol. Sci. 193: 137-157.
- CHEN, H.-J., 1995 Functional domains of the doublesex proteins in Drosophila melanogaster. Ph.D. Thesis, University of California, Davis.
- HENIKOFF, S., M. A. KEENE, J. S. SLOAN, J. BLESKAN, R. HARD^ 1986b Multiple purine pathway enzyme activities are encoded at a single genetic locus. Proc. Natl. Acad. Sci. USA 83: 720-724.
- MANIATIS, T., E. F. FKITSCH andJ. SAMBROOK, 1982 Molecular Clon- ing. Cold Spring Harbor Laboratory Press, Cold Spring Har- bor, N Y . MICHEISON, A. M., S. M. ABMAYR, M. BATE, A. MARTINEZ-ARIAS and T. MANIATIS, 1990 Expression of a MyoD family member pre- figures muscle pattern in Drosophila embryos. Genes Dev. 4 2086-2097.
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Self-citationWang, W., Zhang, J., Alvarez, C., Llopart, A., & Long, M. (2000) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a026413 · Cited by 103
Functional unknomics: Systematic screening of conserved genes of unknown function
Rocha, J. J., Jayaram, S. A., Stevens, T. J., Muschalik, N., Shah, R. D., Emran, S., Robles, C., Freeman, M., & Munro, S. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002222 · Cited by 70
Genetic Organization of Polytene Chromosomes
Zhimulev, I. F. (1999) · Advances in genetics · doi:10.1016/s0065-2660(08)60476-9 · Cited by 58
Natural alcohol exposure: Is ethanol the main substrate for alcohol dehydrogenases in animals?
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Ivanov, I. P., Simin, K. J., Letsou, A., Atkins, J. F., & Gesteland, R. F. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.3.1553 · Cited by 34
Taylor, C. A., Stanley, K. N., & Shirras, A. D. (1997) · Gene · doi:10.1016/s0378-1119(97)00429-0 · Cited by 25
A Nested Alpha-Amylase Gene in Drosophila ananassae
Lage, J. D., Maisonhaute, C., Maczkowiak, F., & Cariou, M. L. (2003) · Journal of Molecular Evolution · doi:10.1007/s00239-003-2488-4 · Cited by 14
Functional unknomics: closing the knowledge gap to accelerate biomedical researchPreprint
Rocha, J., Jayaram, S. A., Stevens, T. J., Muschalik, N., Shah, R. D., Emran, S., Robles, C., Freeman, M., & Munro, S. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.06.28.497983 · Cited by 4
Concordant developmental expression profiles of orthologues in highly divergent BilateriaPreprint
Luca Ferretti, A., Krämer-Eis, A., & Schiffer, P. H. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/786723
Metadata
- Authors
- McNabb, S., Greig, S., & Davis, T.
- Affiliations
- University of Cambridge (GB)
- Corresponding author
- McNabb, S
- ORCID
- Davis, T
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-06-01
- Volume
- 143
- Issue
- 2
- Pages
- 897-911
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1850375718
- Cited by
- 10
- References
- 0
- Citations by year
- 2023: 1 · 2022: 1 · 2019: 1
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- alcohol dehydrogenase gene, ADH, Drosophila melanogaster, chromosomal breakpoints, alternative splicing, cDNA cloning, maternal and zygotic gene expression, nurse cells, oocytes, myogenesis
- MeSH terms
- Alcohol Dehydrogenase, Animals, Chromosome Mapping, Drosophila melanogaster, Mutation, RNA, Restriction Mapping, Polymerase Chain Reaction, In Situ Hybridization, Gene Expression Regulation, Developmental
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