Analysis of site-specific N-glycan remodeling in the endoplasmic reticulum and the Golgi
Open accessAbstract
The hallmark of N-linked protein glycosylation is the generation of diverse glycan structures in the secretory pathway. Dynamic, non-template-driven processes of N-glycan remodeling in the endoplasmic reticulum and the Golgi provide the cellular setting for structural diversity. We applied newly developed mass spectrometry-based analytics to quantify site-specific N-glycan remodeling of the model protein Pdi1p expressed in insect cells. Molecular dynamics simulation, mutational analysis, kinetic studies of in vitro processing events and glycan flux analysis supported the defining role of the protein in N-glycan processing.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Glycosylation sites can vary in accessibility and the surrounding amino acid environment on the surface of a protein.
“Glycosylation sites can vary both in accessibility and the surrounding amino acid environment on the surface of a protein [109].”
From Glycoengineering Chinese hamster ovary cells: a short history (2021) · cites this paper as [109]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
North, S. J., Huang, H.-H., Sundaram, S., Jang-Lee, J., Etienne, A. T., Trollope, A., Chalabi, S., Dell, A., Stanley, P., & Haslam, S. M. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.068353 · Cited by 207 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Visualization of the HIV-1 Env glycan shield across scales
Berndsen, Z. T., Chakraborty, S., Wang, X., Cottrell, C. A., Torres, J. L., Diedrich, J. K., López, C. A., Yates, J. R., van Gils, M. J., Paulson, J. C., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2000260117 · Cited by 86 · see citing works
Glycoengineering Chinese hamster ovary cells: a short history
Donini, R., Haslam, S. M., & Kontoravdi, C. (2021) · Biochemical Society Transactions · doi:10.1042/bst20200840 · Cited by 37 · see citing works
- Authors
- Hang, I., Lin, C.-W., Grant, O. C., Fleurkens, S., Villiger, T. K., Soos, M., Morbidelli, M., Woods, R. J., Gauss, R., & Aebi, M.
- Affiliations
- Institute of Microbiology (CN)
- University of Georgia (US)
- ETH Zurich (CH)
- Corresponding author
- Gauss, Robert
- Aebi, Markus
- Venue
- Glycobiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-08-03
- Volume
- 25
- Issue
- 12
- Pages
- 1335-1349
- ISSN
- 0959-6658 (ISSN-L)
- 1460-2423
- PMC
- PMC4634314
- OpenAlex
- W2218010822
- Cited by
- 67
- References
- 1
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 6 · 2023: 4 · 2022: 3 · 2021: 10 · 2020: 10 · 2019: 5 · 2018: 5 · 2017: 5 · 2016: 12 · 2015: 1
- FWCI
- 3.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Glycobiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- reroDoc Digital Library (submittedVersion)
- Repository for Publications and Research Data (ETH Zurich) (submittedVersion, other-oa)
- ETH Zürich Research Collection
- Funders
- National Institutes of Health (P41GM103390, R01GM094919)
- Science Foundation of Ireland (08/IN.1/B2070)
- ETH Research (07 10-1)
- Swiss National Science Foundation (31003A_127098)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- N-glycosylation, endoplasmic reticulum, Golgi apparatus, glycan processing, secretory pathway, insect cell expression, molecular dynamics simulation, mutation analysis
- MeSH terms
- Animals, Endoplasmic Reticulum, Glycosylation, Golgi Apparatus, Polysaccharides, Protein Processing, Post-Translational, Spodoptera, Protein Disulfide-Isomerases, Saccharomyces cerevisiae Proteins, Sf9 Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
