Assessment of acrosome and nuclear abnormalities in human spermatozoa with large vacuoles
Abstract
Background Spermatozoa with large vacuoles (SLV) may have a negative impact on embryo development. The origin of these vacuoles is unknown. We evaluated acrosome and nucleus alterations in isolated SLV, versus unselected spermatozoa. Methods We studied 20 patients with teratozoospermia. Spermatozoa from the native semen sample and spermatozoa presenting a vacuole occupying >13.0% total head area, isolated under high magnification (×6600), were assessed. Confocal and transmission electron microscope evaluations were performed on SLV and native sperm, respectively. Acrosome morphology and DNA fragmentation were analysed using proacrosin immunolabelling (monoclonal antibody 4D4) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling assay. Chromatin condensation was evaluated with aniline blue staining. Sperm aneuploidy was assessed using fluorescence in situ hybridization. Results SLV represented 38.0 ± 5.10% of motile spermatozoa obtained after gradient density centrifugation. Vacuoles were mainly in the anterior and median sperm head (45.7 ± 2.90 and 46.1 ± 3.00%, respectively). Abnormal acrosomes were increased in SLV compared with unselected spermatozoa (77.8 ± 2.49 versus 70.6 ± 2.62%; P = 0.014). Microscopic observations showed an exclusively nuclear localization of large vacuoles. Complete DNA fragmentation was higher in native spermatozoa (P Conclusions Sperm vacuoles were exclusively nuclear. In our selected teratozoospermic population, aneuploidy a…
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A defect in sperm morphology significantly correlates with global spermatogenic damage.
“A defect in sperm morphology significantly correlates with global spermatogenic damage (72).”
From Isolated teratozoospermia: revisiting its relevance in male infertility: a narrative review (2024) · cites this paper as [72]
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Isolated teratozoospermia: revisiting its relevance in male infertility: a narrative review
Atmoko, W., Savira, M., Shah, R., Chung, E., & Agarwal, A. (2024) · Translational Andrology and Urology · doi:10.21037/tau-23-397 · Cited by 23 · see citing works
“A defect in sperm morphology significantly correlates with global spermatogenic damage (72).”
- Authors
- Perdrix, A., Travers, A., Chelli, M. H., Escalier, D., Do Rego, J. L., Milazzo, J. P., Mousset-Simeon, N., Mace, B., & Rives, N.
- Affiliations
- Cecos (FR)
- Inserm (FR)
- Corresponding author
- Rives, N.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-11-18
- Volume
- 26
- Issue
- 1
- Pages
- 47-58
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2159377832
- Cited by
- 137
- References
- 0
- Citations by year
- 2025: 5 · 2024: 5 · 2023: 2 · 2022: 3 · 2021: 6 · 2020: 5 · 2019: 7 · 2018: 6 · 2017: 11 · 2016: 7 · 2015: 9 · 2014: 18 · 2013: 23 · 2012: 20
- FWCI
- 19.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- acrosome abnormalities, teratozoospermia, sperm DNA fragmentation, chromatin condensation, sperm aneuploidy, sperm diploidy, impaired spermatogenesis, embryonic development
- MeSH terms
- Acrosome, Adult, Aneuploidy, Cell Nucleus, Chromatin, Humans, Infertility, Male, Male, Middle Aged, Spermatozoa, Vacuoles, In Situ Hybridization, Fluorescence, In Situ Nick-End Labeling, Microscopy, Electron, Transmission, Embryonic Development, DNA Fragmentation, Semen Analysis
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