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Fluorometric Determination of Chlorophyll
Abstract
The concentration of chlorophyll in laboratory grown cultures of marine phytoplankton and in oceanic samples has been determined both by measurement of fluorescence and by measurement of light absorption. The lower limit for detection of chlorophyll by fluorescence with the instrumentation described is about 0·01 μg chlorophyll a, which is about 5% that required for a spectro-photometric determination. Through choice of appropriate filters, the amount of fluorescence reflects either the chlorophyll a concentration or the sum of chlorophylls a and c. By measurement of fluorescence before and after acidification, the ratio of chlorophyll to phaeophytin can be readily determined. Dilute HCl is superior to oxalic acid for acidification of pigment extracts. As the fluorometric determination of chlorophyll and phaeophytin is fast, reliable and sensitive, it will be very useful in field studies of productivity.
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Cited by
A fluorometric method for the differentiation of algal populations in vivo and in situ
Beutler, M., Wiltshire, K. H., Meyer, B., Moldaenke, C., Lüring, C., Meyerhöfer, M., Hansen, U.-P., & Dau, H. (2002) · Photosynthesis Research · doi:10.1023/a:1016026607048 · Cited by 560
Photosynthetic pigments in diatoms
Kuczynska, P., Jemiola-Rzeminska, M., & Strzalka, K. (2015) · Marine Drugs · doi:10.3390/md13095847 · Cited by 428
Greg Mitchell, B., & Kiefer, D. A. (1988) · Deep Sea Research Part A. Oceanographic Research Papers · doi:10.1016/0198-0149(88)90024-6 · Cited by 295
Cardona, E., Gueguen, Y., Magré, K., Lorgeoux, B., Piquemal, D., Pierrat, F., Noguier, F., & Saulnier, D. (2016) · BMC Microbiology · doi:10.1186/s12866-016-0770-z · Cited by 267
Ozer, T., Gertman, I., Kress, N., Silverman, J., & Herut, B. (2017) · Global and Planetary Change · doi:10.1016/j.gloplacha.2016.04.001 · Cited by 224
Metadata
- Authors
- Holm-Hansen, O., Lorenzen, C. J., Holmes, R. W., & Strickland, J. D. H.
- Venue
- ICES Journal of Marine Science
- Publisher
- Oxford University Press (OUP)
- Published
- 1965-12-01
- Volume
- 30
- Issue
- 1
- Pages
- 3-15
- ISSN
- 1054-3139 (ISSN-L)
- 1095-9289
- OpenAlex
- W2164689991
- Cited by
- 2,516
- References
- 0
- FWCI
- 18.84
- Type
- journal article
- Language
- en
- Open access
- closed
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- Holm-Hansen, O., Lorenzen, C. J., Holmes, R. W., & Strickland, J. D. H. (1965). Fluorometric Determination of Chlorophyll. ICES Journal of Marine Science. https://doi.org/10.1093/icesjms/30.1.3
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
