CoChat
Sign Up
Cited by 12Antibiotic Resistance in BacteriaBacterial Genetics and BiotechnologyCRISPR and Genetic Engineering

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Transmission barrier of the bla KPC plasmid mediated by type I restriction-modification systems in Escherichia coli

Li, Dan, Li, Pei, Peng, Mingjia, Zhao, Xiaoyu, Jiang, Xiaoying, Wang, Dongliang, Yuan, Yuan, Guo, Qinglan, Wang, Minghua, Xu, Xiaogang, Wang, Minggui·2022·Journal of Antimicrobial Chemotherapy
DOI 10.1093/jac/dkab489
Open this paper in CoChat — free account

Abstract

Background: Transportation of carbapenem-resistant plasmids contributes to carbapenem resistance in Gram-negative bacteria. KPC enzymes are the most clinically important enzymes among carbapenem-resistant Klebsiella pneumoniae, whereas the rate of blaKPC in Escherichia coli is low. The CRISPR-Cas system and restriction-modification system (R-M system) in bacteria defend against invading genomes. Currently, the role of the immune systems in the low rate of KPC-producing E. coli remains unclear. Objectives: We investigated the relationship between immune systems and the low detection rate of blaKPC in E. coli. Methods: We searched for blaKPC among 1039 E. coli whole genomes available in GenBank using nucleotide BLAST. CRISPR-Cas systems and the R-M system were detected in all strains having the ST as blaKPC-positive strains. Nucleotide BLAST was used to search for protospacers on blaKPC plasmids. A conjugation assay was performed to determine whether the R-M system influences the acquisition of blaKPC plasmids by E. coli. Results: ST131 was the dominant ST of KPC-producing E. coli and IncN was the main plasmid type (12/32). CRISPR-Cas systems were frequently present in E. coli carrying blaKPC. Furthermore, CRISPR-Cas systems in E. coli didn’t target plasmids with blaKPC. Type I R-M systems were rare in KPC-producing E. coli, but significantly over-represented in KPC-negative strains. E. coli DH5α with hsdR deletion accepted blaKPC-carrying plasmids, whereas those with hsdR complementation impeded blaKPC-carrying plasmid conjugation. Conclusions: Horizontal transmission of blaKPC occurs among E. coli. The type I R-M system is associated with the defence against blaKPC plasmid transport into E. coli.

Overview

Integrity signals

Registry

Crossref · 10.1093/jac/dkab489

Cites

23 of 23 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 23 references

Cited by

Sorted by citations

See all 12

Related

See all

Metadata

Authors
Li, D., Li, P., Peng, M., Zhao, X., Jiang, X., Wang, D., Yuan, Y., Guo, Q., Wang, M., Xu, X., & Wang, M.
Affiliations
  • Fudan University (CN)
  • Gansu Provincial Hospital (CN)
Corresponding author
Xu, Xiaogang
ORCID
Venue
Journal of Antimicrobial Chemotherapy
Publisher
Oxford University Press (OUP)
Published
2022-01-19
Volume
77
Issue
4
Pages
952-956
ISSN
  • 0305-7453 (ISSN-L)
  • 1460-2091
DOI
10.1093/jac/dkab489
OpenAlex
W4206154257
Cited by
12
References
23
Citations by year
2026: 1 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 1
FWCI
0.71
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Journal of Antimicrobial Chemotherapy (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81402976, 81773785)
  • Science and Technology Commission of Shanghai Municipality (19JC1413002)
Topics
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Keywords
blaKPC, carbapenem resistance, Escherichia coli, type I restriction-modification system, CRISPR-Cas, plasmid conjugation, horizontal gene transfer, ST131, IncN plasmids, Klebsiella pneumoniae, plasmid transmission
MeSH terms
Bacterial Proteins, beta-Lactamases, Escherichia coli, Humans, Klebsiella Infections, Klebsiella pneumoniae, Plasmids, DNA Restriction-Modification Enzymes

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review