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Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L.
Open accessAbstract
The activities of chlorophyllase, contents of pigments including chlorophyll a and b, chlorophyllide a and b, and phaeophorbide a during leaf senescence under low oxygen (0.5% O2) and control (air) were investigated in a non-yellowing mutant and wild-type leaves of snap beans (Phaseolus vulgaris L.). Chlorophyllase from leaf tissues had maximum activity when incubated at 40°C in a mixture containing 50% acetone. In both mutant and wild type, chlorophyllase activity was the highest in freshly harvested non-senescent leaves and decreased sharply in the course of senescence, indicating that the loss of chlorophylls in senescing leaves is not directly related to the activity of chlorophyllase and that chlorophyllase activity is not altered in the mutant. The wild type had higher ratios of chlorophyll a to chlorophyll b than the mutant and chlorophyll a:b ratios increased during senescence in both types. In the senescent mutant leaves, accumulations of chlorophyllide a and chlorophyllide b were detected, but no phaeophorbide a was found. Chlorophyllide b had a greater accumulation than chlorophyllide a in the early stage of senescence. Low oxygen treatment not only delayed chlorophyll degradation but also enhanced the accumulations of chlorophyllide a and b and lowered the ratios of chlorophyll a to chlorophyll b.
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“The salt-induced alterations in a leaf Chl content could be due to impaired biosynthesis or accelerated pigment degradation.However, during the process of Chl degradation, Chl b may be converted into Chl a, thus resulting in the increased content of Chl a (Fang et al. 1998, Eckardt 2009).”
From Photosynthesis under stressful environments: An overview (2013) · cites this paper as Fang et al. 1998
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Research works printed but not linked: 16
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Cited by
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Photosynthesis under stressful environments: An overview
Ashraf, M., & Harris, P. J. C. (2013) · Photosynthetica · doi:10.1007/s11099-013-0021-6 · Cited by 2,028
Matile, P., Hörtensteiner, S., & Thomas, H. (1999) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.50.1.67 · Cited by 515
Metadata
- Authors
- Fang, Z., Bouwkamp, J. C., & Solomos, T.
- Affiliations
- University of Maryland, College Park (US)
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-03-01
- Volume
- 49
- Issue
- 320
- Pages
- 503-510
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W2107925063
- Cited by
- 259
- References
- 0
- Citations by year
- 2025: 11 · 2024: 9 · 2023: 15 · 2022: 15 · 2021: 19 · 2020: 14 · 2019: 16 · 2018: 9 · 2017: 20 · 2016: 18 · 2015: 17 · 2014: 12 · 2013: 10 · 2012: 10
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Experimental Botany (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
- Keywords
- chlorophyllase, chlorophyll degradation, leaf senescence, Phaseolus vulgaris, green beans, chlorophyllide a, phaeophorbide a, chlorophyll a/b ratio, hypoxia
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- CoChat. (2026). Citation check: Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L. https://app.cochat.ai/papers/10.1093/jxb/49.320.503
- The paper
- Fang, Z., Bouwkamp, J. C., & Solomos, T. (1998). Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L. Journal of Experimental Botany. https://doi.org/10.1093/jxb/49.320.503
