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In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen‐inducible PR 10 promoter
Open accessAbstract
Resveratrol is a major phytoalexin in grapevine but its synthesis in response to phytopathogen attack decreases with grape berry ripening. A chimeric gene combining an alfalfa PR 10 promoter and Vst1 (Vitis stilbene synthase 1) gene was introduced into the genome of 41B rootstock. Transgenic plants were analysed for resveratrol production in leaves infected with Botrytis using an in vitro test. Among the 50 transgenic lines analysed, some exhibited a production lower than the non-transgenic control, but others accumulated resveratrol from 5-100-fold. Moreover, in the latter clones, symptoms were highly reduced in response to infection. These results were a good indication that the combination of a pathogen-inducible promoter and a defence gene may increase tolerance against fungi in grapevine. The efficacy of this approach should be further tested by experiments conducted in the vineyard.
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Citations
Cited by
Metabolism and roles of stilbenes in plants
Chong, J., Poutaraud, A., & Hugueney, P. (2009) · Plant Science · doi:10.1016/j.plantsci.2009.05.012 · Cited by 664
Grapevine trunk diseases: complex and still poorly understood
Bertsch, C., Ramírez‐Suero, M., Magnin‐Robert, M., Larignon, P., Chong, J., Abou‐Mansour, E., Spagnolo, A., Clément, C., & Fontaine, F. (2013) · Plant Pathology · doi:10.1111/j.1365-3059.2012.02674.x · Cited by 451
Jeandet, P., Delaunois, B., Conreux, A., Donnez, D., Nuzzo, V., Cordelier, S., Clément, C., & Courot, E. (2010) · BioFactors · doi:10.1002/biof.108 · Cited by 266
Aziz, A., Trotel-Aziz, P., Dhuicq, L., Jeandet, P., Couderchet, M., & Vernet, G. (2006) · Phytopathology® · doi:10.1094/phyto-96-1188 · Cited by 260
Metadata
- Authors
- Coutos‐Thévenot, P., Poinssot, B., Bonomelli, A., Yean, H., Breda, C., Buffard, D., Esnault, R., Hain, R., & Boulay, M.
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-05-01
- Volume
- 52
- Issue
- 358
- Pages
- 901-910
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W2155672261
- Cited by
- 187
- References
- 0
- FWCI
- 7.51
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen‐inducible PR 10 promoter. https://app.cochat.ai/papers/10.1093/jexbot/52.358.901
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- Coutos‐Thévenot, P., Poinssot, B., Bonomelli, A., Yean, H., Breda, C., Buffard, D., Esnault, R., Hain, R., & Boulay, M. (2001). In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen‐inducible PR 10 promoter. Journal of Experimental Botany. https://doi.org/10.1093/jexbot/52.358.901
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