Expression of the Nicotiana protein kinase (NPK1) enhanced drought tolerance in transgenic maize.
Open accessAbstract
Drought is one of the most important abiotic stresses affecting the productivity of maize. Previous studies have shown that expression of a mitogen-activated protein kinase kinase kinase (MAPKKK) gene activated an oxidative signal cascade and led to the tolerance of freezing, heat, and salinity stress in transgenic tobacco. To analyse the role of activation of oxidative stress signalling in improving drought tolerance in major crops, a tobacco MAPKKK (NPK1) was expressed constitutively in maize. Results show that NPK1 expression enhanced drought tolerance in transgenic maize. Under drought conditions, transgenic maize plants maintained significantly higher photosynthesis rates than did the non-transgenic control, suggesting that NPK1 induced a mechanism that protected photosynthesis machinery from dehydration damage. In addition, drought-stressed transgenic plants produced kernels with weights similar to those under well-watered conditions, while kernel weights of drought-stressed non-transgenic control plants were significantly reduced when compared with their non-stressed counterparts.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Constitutive expression of tobacco MAPKKK NPK1 in maize resulted in a transgenic line with enhanced drought tolerance measured in terms of kernel mass and PN.
“Shou et al. (2004) have shown that the tobacco MAPKKK (NPK1) expressed constitutively in maize resulted in a maize transgenic line with enhanced drought tolerance trait measured in terms of kernel mass and P N .”
From Photosynthesis under stressful environments: An overview (2013) · cites this paper as Shou et al. 2004
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Fischer, R. A., Rees, D., Sayre, K. D., Lu, Z. ‐., Condon, A. G., & Saavedra, A. L. (1998) · Crop Science · doi:10.2135/cropsci1998.0011183x003800060011x · Cited by 887 · cited 1× · see citing works
“However, the positive correlation between them has been reported in several studies (Chandra Babu et al., 1985; Fischer et al., 1998; Pettigrew and Meredith, 1994; Pooter and Remkes, 1990; Zelitch, 1982).”
Background
Flagged first, then by citations
Research works printed but not linked: 13
- Carrington JC, Freed DD. 1990. Cap-independent enhancement of translation by a plant potyvirus 5¢ non-translated region. Journal of Virology 64, 1590±1597.
- Debel K, Eberhard D, Kloppstech K. 1995. Light-stress: its effect on the expression of small organellar heat-shock proteins in plants. Clues to their function? In: Leigh RA, Metchteld Blake- Kalff MA, eds. Proceedings of the second STRESSNET Conference. European Commission: Directorate General VI, 29±34.
- Depicker A, Stachel S, Dhaese P, Zambryski P, Goodman HM. 1982. Nopaline synthase: transcript mapping and DNA sequence. Journal of Molecular and Applied Genetics 1, 561±573.
- Fagard M, Vaucheret H. 2000. (Trans)gene silencing in plants: how many mechanisms? Annual Review of Plant Physiology and Plant Molecular Biology 51, 167±194.
- Ishikawa M, Soyano T, Nishihama R, Machida Y. 2002. The NPK1 mitogen-activated protein kinase kinase kinase contains a functional nuclear localization signal at the binding site for the NACK1 kinesin-like protein. The Plant Journal 32, 789±798.
- Kovtun Y, Chiu WL, Tena G, Sheen J. 2000. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proceedings of the National Academy of Sciences,USA 97, 2940±2945.
- Li HS, Chang CS, Lu LS, Liu CA, Chan MT, Charng YY. 2003. Over-expression of Arabidopsis thaliana heat shock factor gene (AtHsf1b) enhances chilling tolerance in transgenic tomato. Botanic Bulletin of Academia Sinica 44, 129±140.
- Pararajasingham S, Hunt LA. 1996. Effects of photoperiod on leaf appearance rate and leaf dimensions in winter and spring wheats. Canadian Journal of Plant Science 76, 43±50.
- Ristic Z, Yang GP, Martin B, Fullerton S. 1998. Evidence of association between speci®c heat-shock protein(s) and the drought and heat tolerance phenotype in maize. Journal of Plant Physiology 153, 497±505.
- Samarah NH. 2000. Soybean yield, yield components, seed quality, dehydrin-like proteins, soluble sugars, and mineral nutrients in response to drought stress imposed prior to severe stress. PhD thesis, Iowa State University, Ames, IA, 64±65.
- Sheen J. 1993. Protein phosphatase activity, is required for light- inducible gene expression in maize. EMBO Journal 12, 3497± 3505.
- Shou H, Bordallo P, Fan JB, Yeakley JM, Bibikova M, Sheen J, Wang K. 2004a. Expression of an active tobacco mitogen- activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize. Proceedings of the National Academy of Sciences, USA 101, 3298±3303.
- Thompson CK, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, Botterman J. 1987. Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO Journal 6, 2519±2523.
Cited by
Sorted by citations
Photosynthesis under stressful environments: An overview
Ashraf, M., & Harris, P. J. C. (2013) · Photosynthetica · doi:10.1007/s11099-013-0021-6 · Cited by 2,028 · see citing works
Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future
Umezawa, T., Fujita, M., Fujita, Y., Yamaguchi-Shinozaki, K., & Shinozaki, K. (2006) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2006.02.002 · Cited by 802 · see citing works
Abiotic Stress Responses in Plants: Unraveling the Complexity of Genes and Networks to Survive
Sofia, A., de Almeida, A. M., da Silva, A. B., da Silva, J. M., Paula, A., Santos, D., Fevereiro, P., & Sousa Araujo, S. D. (2013) · Abiotic Stress - Plant Responses and Applications in Agriculture · doi:10.5772/52779 · Cited by 106 · see citing works
- Authors
- Shou, H.
- Affiliations
- Iowa State University (US)
- Corresponding author
- Shou, H.
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-04-08
- Volume
- 55
- Issue
- 399
- Pages
- 1013-1019
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W2126947140
- Cited by
- 250
- References
- 1
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 11 · 2022: 16 · 2021: 8 · 2020: 12 · 2019: 9 · 2018: 11 · 2017: 10 · 2016: 11 · 2015: 9 · 2014: 14 · 2013: 18 · 2012: 10
- FWCI
- 12.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Experimental Botany (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0077692)
- Shanghai Ocean University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetics and Plant Breeding
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- MAP3K, protein kinases, drought tolerance, transgenic maize, photosynthesis, grain weight, abiotic stress
- MeSH terms
- Zea mays, Disasters, Photosynthesis, Plant Proteins, Seeds, Nicotiana, Water, Restriction Mapping, Signal Transduction, Polymerase Chain Reaction, Plant Leaves, MAP Kinase Kinase Kinases, Plants, Genetically Modified
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
