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Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content
Open accessAbstract
Top-down control analysis (TDCA) is a useful tool for quantifying constraints on metabolic pathways that might be overcome by biotechnological approaches. Previous studies on lipid accumulation in oilseed rape have suggested that diacylglycerol acyltransferase (DGAT), which catalyses the final step in seed oil biosynthesis, might be an effective target for enhancing seed oil content. Here, increased seed oil content, increased DGAT activity, and reduced substrate:product ratio are demonstrated, as well as reduced flux control by complex lipid assembly, as determined by TDCA in Brassica napus (canola) lines which overexpress the gene encoding type-1 DGAT. Lines overexpressing DGAT1 also exhibited considerably enhanced seed oil content under drought conditions. These results support the use of TDCA in guiding the rational selection of molecular targets for oilseed modification. The most effective lines had a seed oil increase of 14%. Moreover, overexpression of DGAT1 under drought conditions reduced this environmental penalty on seed oil content.
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Cites
Metabolic control analysis: a survey of its theoretical and experimental development
Fell, D. A. (1992) · Biochemical Journal · doi:10.1042/bj2860313 · Cited by 782
Cited by
Advances in the Production of High-Value Products by Microalgae
Leu, S., & Boussiba, S. (2014) · Industrial Biotechnology · doi:10.1089/ind.2013.0039 · Cited by 253
Bellou, S., & Aggelis, G. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.01.010 · Cited by 189
Algal Lipids and Their Metabolism
Guschina, I. A., & Harwood, J. L. (2013) · Algae for Biofuels and Energy · doi:10.1007/978-94-007-5479-9_2 · Cited by 83
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Metadata
- Authors
- Weselake, R. J., Shah, S., Tang, M., Quant, P. A., Snyder, C. L., Furukawa-Stoffer, T. L., Zhu, W., Taylor, D. C., Zou, J., Kumar, A., Hall, L., Laroche, A., Rakow, G., Raney, P., Moloney, M. M., & Harwood, J. L.
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-08-13
- Volume
- 59
- Issue
- 13
- Pages
- 3543-3549
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W2162690480
- Cited by
- 196
- References
- 1
- FWCI
- 4.12
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Weselake, R. J., Shah, S., Tang, M., Quant, P. A., Snyder, C. L., Furukawa-Stoffer, T. L., Zhu, W., Taylor, D. C., Zou, J., Kumar, A., Hall, L., Laroche, A., Rakow, G., Raney, P., Moloney, M. M., & Harwood, J. L. (2008). Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content. Journal of Experimental Botany. https://doi.org/10.1093/jxb/ern206
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
