Cloning and molecular characterization of a mitogen-activated protein kinase gene from Poncirus trifoliata whose ectopic expression confers dehydration/drought tolerance in transgenic tobacco
Open accessAbstract
The mitogen-activated protein kinase (MAPK) cascade plays pivotal roles in diverse signalling pathways related to plant development and stress responses. In this study, the cloning and functional characterization of a group-I MAPK gene, PtrMAPK, in Poncirus trifoliata (L.) Raf are reported. PtrMAPK contains 11 highly conserved kinase domains and a phosphorylation motif (TEY), and is localized in the nucleus of transformed onion epidermal cells. The PtrMAPK transcript level was increased by dehydration and cold, but was unaffected by salt. Transgenic overexpression of PtrMAPK in tobacco confers dehydration and drought tolerance. The transgenic plants exhibited better water status, less reactive oxygen species (ROS) generation, and higher levels of antioxidant enzyme activity and metabolites than the wild type. Interestingly, the stress tolerance capacity of the transgenic plants was compromised by inhibitors of antioxidant enzymes. In addition, overexpression of PtrMAPK enhanced the expression of ROS-related and stress-responsive genes under normal or drought conditions. Taken together, these data demonstrate that PtrMAPK acts as a positive regulator in dehydration/drought stress responses by either regulating ROS homeostasis through activation of the cellular antioxidant systems or modulating transcriptional levels of a variety of stress-associated genes.
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MAP kinases are involved in the mechanisms of tolerance to a variety of biotic and abiotic stresses in plants.
“There is strong evidence now that MAP kinases are involved in the mechanisms of tolerance to a variety of biotic and abiotic stresses in plants (Huang et al. 2011, Zhang et al. 2011).”
From Photosynthesis under stressful environments: An overview (2013) · cites this paper as Huang et al. 2011
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Photosynthesis under stressful environments: An overview
Ashraf, M., & Harris, P. J. C. (2013) · Photosynthetica · doi:10.1007/s11099-013-0021-6 · Cited by 2,028 · see citing works
- Authors
- Huang, X.-S., Luo, T., Fu, X.-Z., Fan, Q.-J., & Liu, J.-H.
- Affiliations
- Huazhong Agricultural University (CN)
- Corresponding author
- Liu, Ji-Hong
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-07-21
- Volume
- 62
- Issue
- 14
- Pages
- 5191-5206
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- PMC
- PMC3193021
- OpenAlex
- W2151795323
- Cited by
- 112
- References
- 1
- Citations by year
- 2025: 5 · 2024: 9 · 2023: 3 · 2022: 10 · 2021: 6 · 2020: 9 · 2019: 6 · 2018: 5 · 2017: 11 · 2016: 7 · 2015: 17 · 2014: 10 · 2013: 9 · 2012: 4
- FWCI
- 9.26
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Journal of Experimental Botany (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30921002, 31071778, 2011AA100205, 2009CDA080, 30871685)
- Natural Science Foundation of Hubei Province (2011AA100205, 30921002, 2009CDA080)
- Fok Ying Tong Education Foundation (114034)
- National High-tech Research and Development Program (2011AA100205)
- Specialized Research Fund for the Doctoral Program of Higher Education of China (20090146110010)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Keywords
- mitogen-activated protein kinase, Poncirus trifoliata, drought tolerance, dehydration stress, transgenic tobacco, ectopic expression, reactive oxygen species, antioxidant enzymes, cold stress, salt stress, stress-responsive gene expression, MAPK signaling, kinase domain
- MeSH terms
- Amino Acid Sequence, Cloning, Molecular, Molecular Sequence Data, Phylogeny, Plant Proteins, Plants, Nicotiana, Gene Expression, Sequence Alignment, Gene Expression Regulation, Plant, Mitogen-Activated Protein Kinases, Plants, Genetically Modified, Poncirus, Droughts
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