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Cited by 3Venomous Animal Envenomation and StudiesChromosomal and Genetic VariationsEvolution and Genetic Dynamics

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Tandem Duplication of Serpin Genes Yields Functional Variation and Snake Venom Inhibitors

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Ward, Meilyn S, Holding, Matthew L, Haynes, Laura M, Margres, Mark J, Matocq, Marjorie D, Ginsburg, David·2025·Molecular Biology and Evolution
DOI 10.1093/molbev/msaf290PMC PMC11741322

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Abstract

Tandem duplication of genes can play a critical role in the evolution of functional novelty, yet our understanding is limited concerning the role of gene duplication in coevolution between species. Much is known about the evolution and function of tandemly duplicated snake venom genes, however, the potential of gene duplication to fuel venom resistance within prey species is poorly understood. The SERPINA subfamily of genes produces globular serine protease inhibitors and carrier molecules, and SERPINA1 has previously been shown to inhibit snake venom serine proteases. In this study, we characterize patterns of duplication within the tandem array of SERPINA, documenting trends in copy number evolution between species. We find the hallmarks of rapid birth-death evolution of SERPINA1-like and SERPINA3-like genes within and between rodent lineages, and evidence for diversifying selection acting on rodent genes. To explore the functional significance of copy number evolution, we recombinantly expressed the full set of 12 paralogous duplicates of SERPINA3 found in the genome of the Big-eared woodrat (Neotoma macrotis), a species known for resistance to protease-rich rattlesnake venoms. Two SERPINA3 paralogs inhibited venom serine proteases, indicating that these proteins may serve as resistance factors. In addition, functional variation is apparent among paralogs, including neofunctionalization to inhibit both chymotrypsin-like and trypsin-like proteases simultaneously for one venom-inhibiting paralog. Our results provide further evidence that the rapid evolution of SERPINA1 and SERPINA3 gene copy number across rodents has adaptive potential by producing functionally diverse venom inhibitors.

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Metadata

Authors
Ward, M. S., Holding, M. L., Haynes, L. M., Margres, M. J., Matocq, M. D., & Ginsburg, D.
Affiliations
  • University of Michigan (US)
  • University of South Florida (US)
  • University of Nevada, Reno (US)
Corresponding author
Holding, Matthew L
ORCID
Venue
Molecular Biology and Evolution
Publisher
Oxford University Press (OUP)
Published
2025-10-29
Volume
42
Issue
11
ISSN
  • 0737-4038 (ISSN-L)
  • 1537-1719
DOI
10.1093/molbev/msaf290
PMC
PMC11741322
OpenAlex
W4416665694
Cited by
3
References
1
Citations by year
2026: 2 · 2025: 1
FWCI
1.76
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecular Biology and Evolution (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by-nc-nd)
Funders
  • National Institutes of Health (1r35hl171421-01)
  • National Heart, Lung, and Blood Institute (R35 HL171421)
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Venomous Animal Envenomation and Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
Keywords
tandem gene duplication, SERPINA1, alpha-1-antichymotrypsin, copy number evolution, diversifying selection, neofunctionalization, Neotoma macrotis, rattlesnake venom, paralogs

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