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Polymer-stimulated ligation: enhanced blunt- Or cohesive-end ligation of DNA or deoxyribooligonudcleotides by T4 DNA Ugase in polymer solutions
Open accessAbstract
The rates of blunt-end and cohesive-end ligation of DNA by T4 DNA ligase are increased by orders of magnitude in the presence of high concentrations of a variety of nonspecific polymers such as polyethylene glycol, Ficoll, bovine plasma albumin, or glycogen. Blunt-end ligation of small self-complementary oligodeoxyribonucleotides is also stimulated. The use of polyethylene glycol 6000 in such systems is characterized in some detail. Conditions are suggested which either greatly increase the rate of formation of and size of linear ligation products or which allow smaller but significant improvements in the amounts of circular ligation products.
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Citations
Cited by
Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector
Fürste, J. P., Pansegrau, W., Frank, R., Blöcker, H., Scholz, P., Bagdasarian, M., & Lanka, E. (1986) · Gene · doi:10.1016/0378-1119(86)90358-6 · Cited by 1,045
Effects of Molecular Crowding on the Structures, Interactions, and Functions of Nucleic Acids
Nakano, S.-I., Miyoshi, D., & Sugimoto, N. (2014) · Chemical Reviews · doi:10.1021/cr400113m · Cited by 567
Acceleration of nucleic acid hybridization rate by polyethylene glycol
Amasino, R. M. (1986) · Analytical Biochemistry · doi:10.1016/0003-2697(86)90413-6 · Cited by 375
Zimmerman, S. B. (1993) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(93)90142-z · Cited by 107
Self-citationMurphy, L. D., & Zimmerman, S. B. (1995) · Biophysical Chemistry · doi:10.1016/0301-4622(95)00047-2 · Cited by 90
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Metadata
- Authors
- Pheiffer, B. H., & Zimmerman, S. B.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1983
- Volume
- 11
- Issue
- 22
- Pages
- 7853-7871
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2075031558
- Cited by
- 171
- References
- 0
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Polymer-stimulated ligation: enhanced blunt- Or cohesive-end ligation of DNA or deoxyribooligonudcleotides by T4 DNA Ugase in polymer solutions. https://app.cochat.ai/papers/10.1093/nar/11.22.7853
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- Pheiffer, B. H., & Zimmerman, S. B. (1983). Polymer-stimulated ligation: enhanced blunt- Or cohesive-end ligation of DNA or deoxyribooligonudcleotides by T4 DNA Ugase in polymer solutions. Nucleic Acids Research. https://doi.org/10.1093/nar/11.22.7853
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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