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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
Open accessAbstract
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).
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Cited by
Nan, X., Ng, H.-H., Johnson, C. A., Laherty, C. D., Turner, B. M., Eisenman, R. N., & Bird, A. (1998) · Nature · doi:10.1038/30764 · Cited by 3,440
Kastan, M. B., Zhan, Q., El-Deiry, W. S., Carrier, F., Jacks, T., Walsh, W. V., Plunkett, B. S., Vogelstein, B., & Fornace, A. J. (1992) · Cell · doi:10.1016/0092-8674(92)90593-2 · Cited by 3,047
Multiple nuclear factors interact with the immunoglobulin enhancer sequences
Sen, R., & Baltimore, D. (1986) · Cell · doi:10.1016/0092-8674(86)90346-6 · Cited by 2,752
Semenza, G. L., & Wang, G. L. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.12.5447 · Cited by 2,617
Auphan, N., DiDonato, J. A., Rosette, C., Helmberg, A., & Karin, M. (1995) · Science · doi:10.1126/science.270.5234.286 · Cited by 2,390
Metadata
- Authors
- Dignam, J. D., Lebovitz, R. M., & Roeder, R. G.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1983-03-11
- Volume
- 11
- Issue
- 5
- Pages
- 1475-1489
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1993243592
- Cited by
- 11,260
- References
- 0
- FWCI
- 21.62
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. https://app.cochat.ai/papers/10.1093/nar/11.5.1475
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- Dignam, J. D., Lebovitz, R. M., & Roeder, R. G. (1983). Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Research. https://doi.org/10.1093/nar/11.5.1475
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
