The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Molecular cloning of mouse tumour necrosis factor cDNA and its eukaryotic expression
Open accessAbstract
Tumour necrosis factor (TNF), released by induced macrophages, causes tumour necrosis in animals and kills preferentially transformed cells in vitro. mRNA induced in the established mouse monocytic PU 5.1.8 cell line by lipopolysaccharide, was converted into double-stranded cDNA and cloned in the pAT153 vector. Recombinant plasmids were screened by plus-minus hybridization and TNF-specific oligonucleotide probes constructed on the basis of partial amino acid sequences of rabbit TNF. A series of TNF specific clones were identified and confirmed by hybrid selection of mouse TNF-specific mRNA. The sequence codes for a 235 amino acids long polypeptide, of which 156 amino acids presumably correspond to the mature product. It can be concluded that mature mouse TNF is a glycosylated dimer. Biologically active TNF was secreted by both Cos-I and CHO-cells transfected with the chimaeric expression vector pSV2d2-mTNF containing the coding region of the mouse TNF cDNA gene.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
An endotoxin-induced serum factor that causes necrosis of tumors
Carswell, E. A., Old, L. J., Kassel, R. L., Green, S., Fiore, N., & Williamson, B. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.9.3666 · Cited by 4,532
Cited by
Cachectin and tumour necrosis factor as two sides of the same biological coin
Beutler, B., & Cerami, A. (1986) · Nature · doi:10.1038/320584a0 · Cited by 1,647
Schulze-Osthoff, K., Bakker, A. C., Vanhaesebroeck, B., Beyaert, R., Jacob, W. A., & Fiers, W. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42768-8 · Cited by 939
Self-citationDual Signaling of the Fas Receptor: Initiation of Both Apoptotic and Necrotic Cell Death Pathways
Vercammen, D., Brouckaert, G., Denecker, G., Van de Craen, M., Declercq, W., Fiers, W., & Vandenabeele, P. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.5.919 · Cited by 556
Self-citationSherry, B., & Cerami, A. (1988) · The Journal of cell biology · doi:10.1083/jcb.107.4.1269 · Cited by 432
THORENS, B. (1987) · Cell · doi:10.1016/0092-8674(87)90245-5 · Cited by 353
Metadata
- Authors
- Fransen, L., Mūller, R., Marmenout, A., Tavernier, J., Heyden, J. V. D., Kawashima, E., Chollet, A., Tizard, R., Heuverswyn, H. V., Vliet, A. V., Ruysschaert, M.-R., & Fiers, W.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1985
- Volume
- 13
- Issue
- 12
- Pages
- 4417-4429
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2087436694
- Cited by
- 263
- References
- 2
- FWCI
- 12.57
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Fransen, L., Mūller, R., Marmenout, A., Tavernier, J., Heyden, J. V. D., Kawashima, E., Chollet, A., Tizard, R., Heuverswyn, H. V., Vliet, A. V., Ruysschaert, M.-R., & Fiers, W. (1985). Molecular cloning of mouse tumour necrosis factor cDNA and its eukaryotic expression. Nucleic Acids Research. https://doi.org/10.1093/nar/13.12.4417
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