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Electroporation for the efficient transfection of mammalian cells with DNA

Open access
Chu, Gilbert, Hayakawa, Hiroshi, Berg, Paul·1987·Nucleic Acids Research

Abstract

A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.

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Metadata

Authors
Chu, G., Hayakawa, H., & Berg, P.
Venue
Nucleic Acids Research
Published
1987
Volume
15
Issue
3
Pages
1311-1326
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2056799965
Cited by
832
References
0
FWCI
67.71
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Electroporation for the efficient transfection of mammalian cells with DNA. https://app.cochat.ai/papers/10.1093/nar/15.3.1311
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Chu, G., Hayakawa, H., & Berg, P. (1987). Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/15.3.1311

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.