The DOI matches its Crossref record.
Size fractionation of double-stranded DNA by precipitation with polyethylene glycol
Open accessAbstract
We show that DNA molecules of differing molecular mass are separable by selective precipitation with polyethylene glycol (PEG+.. Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA. Double-stranded DNA can be fractionated at least in the range of 3 times 10-7 to 1 times 10-5 daltons. The effects on PEG concentration, sodium chloride concentration, DNA concentration, pH, divalent ions, precipitation time, and centrifugal force have been determined. These studies show PEG precipitation offers a size fractionation method for DNA which is convenient, of high capacity, and applicable over a wide range of conditions. However, resolution is not high and separation of two species approaches 100% only if they differ in molecular mass by at least a factor of two.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Acceleration of nucleic acid hybridization rate by polyethylene glycol
Amasino, R. M. (1986) · Analytical Biochemistry · doi:10.1016/0003-2697(86)90413-6 · Cited by 375
Oberacker, P., Stepper, P., Bond, D. M., Höhn, S., Focken, J., Meyer, V., Schelle, L., Sugrue, V. J., Jeunen, G.-J., Moser, T., et al. (2019) · PLOS Biology · doi:10.1371/journal.pbio.3000107 · Cited by 293
Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function
Dodonova, S. O., Zhu, F., Dienemann, C., Taipale, J., & Cramer, P. (2020) · Nature · doi:10.1038/s41586-020-2195-y · Cited by 265
Kamiya, M., Kimura, N., Umezawa, N., Hasegawa, H., & Yasuda, S. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1191815 · Cited by 21
Metadata
- Authors
- Lis, J. T., & Schleif, R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1975
- Volume
- 2
- Issue
- 3
- Pages
- 383-390
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2094671300
- Cited by
- 231
- References
- 0
- FWCI
- 0.92
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Size fractionation of double-stranded DNA by precipitation with polyethylene glycol. https://app.cochat.ai/papers/10.1093/nar/2.3.383
- The paper
- Lis, J. T., & Schleif, R. (1975). Size fractionation of double-stranded DNA by precipitation with polyethylene glycol. Nucleic Acids Research. https://doi.org/10.1093/nar/2.3.383
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
