The DOI matches its Crossref record; 10 of the 11 references it cites were found in a registry or an index.
Atomic force microscopy of DNA in aqueous solutions
Open accessAbstract
DNA on mica can be imaged in the atomic force microscope (AFM) in water or in some buffers if the sample has first been dehydrated thoroughly with propanol or by baking in vacuum and if the sample is imaged with a tip that has been deposited in the scanning electron microscope (SEM). Without adequate dehydration or with an unmodified tip, the DNA is scraped off the substrate by AFM-imaging in aqueous solutions. The measured heights and widths of DNA are larger in aqueous solutions than in propanol. The measured lengths of DNA molecules are the same in propanol and in aqueous solutions and correspond to the base spacing for B-DNA, the hydrated form of DNA; when the DNA is again imaged in propanol after buffer, however, it shortens to the length expected for dehydrated A-DNA. Other results include the imaging of E. coli RNA polymerase bound to DNA in a propanol-water mixture and the observation that washing samples in the AFM is an effective way of disaggregating salt-DNA complexes. The ability to image DNA in aqueous solutions has potential applications for observing processes involving DNA in the AFM.
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“AFM has been indicated as a proper tool for micelles size determination due to its ability to distinguish particles with different dimensions in a mixture as well as to operate at room temperature, thus allowing the analysis of biological samples close to native and physiological conditions [133,[154][155][156].”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [155]
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Cites
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Imaging Crystals, Polymers, and Processes in Water with the Atomic Force Microscope
Drake, B., Prater, C. B., Weisenhorn, A. L., Gould, S. A. C., Albrecht, T. R., Quate, C. F., Cannell, D. S., Hansma, H. G., & Hansma, P. K. (1989) · Science · doi:10.1126/science.2928794 · Cited by 1,033
Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with the Atomic Force Microscope
Hansma, H. G., Vesenka, J. P., Siegerist, C. E., Kelderman, G., Morrett, H., Sinsheimer, R. L., Elings, V. B., Bustamante, C., & Hansma, P. K. (1992) · Science · doi:10.1126/science.256.5060.1180 · Cited by 517
Circular DNA molecules imaged in air by scanning force microscopy
Bustamante, C., Vesenka, J. P., Tang, C., Rees, W. A., Guthold, M., & Keller, R. (1992) · Biochemistry · doi:10.1021/bi00116a005 · Cited by 442
Substrate preparation for reliable imaging of DNA molecules with the scanning force microscope
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Atomic force microscopy of single-and double-stranded DNA
Hansma, H. G., Sinsheimer, R. L., Li, M., & Hansma, P. K. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.14.3585 · Cited by 139
Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy
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Imaging of DNA by scanning force microscopy
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Research works printed but not linked: 4
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Cited by
Sorted by citations
Ghezzi, M., Pescina, S., Padula, C., Santi, P., Del Favero, E., Cantù, L., & Nicoli, S. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.02.031 · Cited by 1,135
Tapping mode atomic force microscopy in liquids
Hansma, P. K., Cleveland, J. P., Radmacher, M., Walters, D. A., Hillner, P. E., Bezanilla, M., Fritz, M., Vie, D., Hansma, H. G., Prater, C. B., et al. (1994) · Applied Physics Letters · doi:10.1063/1.111795 · Cited by 876
Biomolecular Imaging with the Atomic Force Microscope
Hansma, H. G., & Hoh, J. H. (1994) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.bb.23.060194.000555 · Cited by 540
Hung, A. M., Micheel, C., Bozano, L. D., Osterbur, L. W., Wallraff, G. M., & Cha, J. N. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.450 · Cited by 432
Visualization of MacromoleculesA First Step to Manipulation and Controlled Response
Sheiko, S. S., & Möller, M. (2001) · Chemical Reviews · doi:10.1021/cr990129v · Cited by 386
Metadata
- Authors
- Hansma, H. G., Bezanilla, M., Zenhausern, F., Adrian, M., & Sinsheimer, R. L.
- Affiliations
- University of California, Santa Barbara (US)
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1993
- Volume
- 21
- Issue
- 3
- Pages
- 505-512
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC309146
- OpenAlex
- W2077943655
- Cited by
- 207
- References
- 11
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 3 · 2023: 7 · 2022: 1 · 2021: 3 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 1 · 2012: 8
- FWCI
- 12.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
- Defense Technical Information Center (DTIC) (submittedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- National Science Foundation (9018846)
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (27142)
- University of California, Santa Barbara
- Office of Naval Research
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- DNA imaging, mica substrate, sample dehydration, DNA hydration, B-DNA, A-DNA, DNA length measurement, RNA polymerase-DNA complex, sample washing
- MeSH terms
- 1-Propanol, DNA, Microscopy, Plasmids, DNA-Directed RNA Polymerases, Solutions, Water
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