The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins
Open accessAbstract
A number of proteins have been rendered functionally oestrogen-dependent by fusion with the hormone-binding domain of the oestrogen receptor. There are, however, several significant disadvantages with such fusion proteins. First, their use in cells in vitro requires phenol red-free medium and laborious stripping of steroid hormones from serum in order to avoid constitutive activation. Secondly, control of oestrogen receptor fusion proteins in vivo is precluded by high endogenous levels of circulating oestrogens. Thirdly, the hormone-binding domain of the oestrogen receptor functions as a hormone-dependent transcriptional activation domain making interpretation of fusions with transcription factors problematical. In order to overcome these drawbacks we have used a transcriptionally inactive mutant of the murine oestrogen receptor which is unable to bind oestrogen yet retains normal affinity for the synthetic ligand, 4-hydroxytamoxifen. When the hormone-binding domain of this mutant oestrogen receptor is fused to the C-terminus of the c-Myc protein, Myc-induced proliferation and apoptosis in fibroblasts becomes dependent on 4-hydroxytamoxifen, but remains refractory to 17 beta-oestradiol.
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Berthois, Y., Katzenellenbogen, J. A., & Katzenellenbogen, B. S. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.8.2496 · Cited by 1,307
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Umek, R. M., Friedman, A. D., & McKnight, S. L. (1991) · Science · doi:10.1126/science.1987644 · Cited by 712
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Metadata
- Authors
- Littlewood, T. D., Hancock, D. C., Danielian, P. S., Parker, M. G., & Evan, G. I.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-05-25
- Volume
- 23
- Issue
- 10
- Pages
- 1686-1690
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2037872087
- Cited by
- 781
- References
- 2
- FWCI
- 11.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Littlewood, T. D., Hancock, D. C., Danielian, P. S., Parker, M. G., & Evan, G. I. (1995). A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins. Nucleic Acids Research. https://doi.org/10.1093/nar/23.10.1686
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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