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Synthesis, deprotection, analysis and purification of RNA and ribosomes
Open accessAbstract
Improvements in the synthesis, deprotection and purification of oligoribonucleotides are described. These advances allow for reduced synthesis and deprotection times, while improving product yield. Coupling times are reduced by half using 5-ethylthio-1H-tetrazole (S-ethyltetrazole) as the activator. Base and 2'-O-t-butyldimethylsilyl deprotection with methylamine (MA) and anhydrous triethylamine/hydrogen fluoride in N-methylpyrrolidinone (TEA.HF/NMP), respectively, requires a fraction of the time necessitated by current standard methods. In addition, the ease of oligoribonucleotide purification and analysis have been significantly enhanced using anion exchange chromatography. These new methods improve the yield and quality of the oligoribonucleotides synthesized. Hammerhead ribozymes synthesized utilizing the described methods exhibited no diminution in catalytic activity.
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Cited by
Xia, T., SantaLucia, J., Burkard, M. E., Kierzek, R., Schroeder, S. J., Jiao, X., Cox, C., & Turner, D. H. (1998) · Biochemistry · doi:10.1021/bi9809425 · Cited by 1,140
Widespread genetic switches and toxicity resistance proteins for fluoride
Baker, J. L., Sudarsan, N., Weinberg, Z., Roth, A., Stockbridge, R. B., & Breaker, R. R. (2012) · Science · doi:10.1126/science.1215063 · Cited by 438
Active Disruption of an RNA-Protein Interaction by a DExH/D RNA Helicase
Jankowsky, E., Gross, C. H., Shuman, S., & Pyle, A. M. (2001) · Science · doi:10.1126/science.291.5501.121 · Cited by 305
Kumar, R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.6.1272 · Cited by 152
Mihaylova, V. T., Kong, Y., Fedorova, O., Sharma, L., Dela Cruz, C. S., Pyle, A. M., Iwasaki, A., & Foxman, E. F. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.08.033 · Cited by 59
Metadata
- Authors
- Wincott, F., DiRenzo, A., Shaffer, C., Grimm, S., Tracz, D., Workman, C., Sweedler, D., Gonzalez, C., Scaringe, S., & Usman, N.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1995
- Volume
- 23
- Issue
- 14
- Pages
- 2677-2684
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W4243831105
- Cited by
- 398
- References
- 0
- FWCI
- 6.99
- Type
- journal article
- Language
- en
- Open access
- green
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- Wincott, F., DiRenzo, A., Shaffer, C., Grimm, S., Tracz, D., Workman, C., Sweedler, D., Gonzalez, C., Scaringe, S., & Usman, N. (1995). Synthesis, deprotection, analysis and purification of RNA and ribosomes. Nucleic Acids Research. https://doi.org/10.1093/nar/23.14.2677
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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