The DOI matches its Crossref record.
Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation
Open accessAbstract
Molecular genetic analysis of isolated single cells and other minute DNA samples is limited because there is insufficient DNA to perform more than one independent PCR amplification. One solution to this problem is to first amplify the entire genome, thus providing enough DNA for numerous subsequent PCRs. In this study we have investigated four different methods of whole genome amplification performed on single cells, and have identified a protocol that generates sufficient quantities of DNA for comparative genomic hybridisation (CGH) as well as more than 90 independent amplification reactions. Thus, numerous specific loci and the copy number of every chromosome can be assessed in a single cell. We report here the first reliable application of CGH to single cells from human preimplantation embryos (blastomeres) and to single fibroblasts, buccal cells and amniocytes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Comprehensive human genome amplification using multiple displacement amplification
Dean, F. B., Hosono, S., Fang, L., Wu, X., Faruqi, A. F., Bray-Ward, P., Sun, Z., Zong, Q., Du, Y., Du, J., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.082089499 · Cited by 1,467
Treff, N. R., Su, J., Tao, X., Levy, B., & Scott, R. T. (2010) · Fertility and Sterility · doi:10.1016/j.fertnstert.2010.01.052 · Cited by 270
The incidence and origin of segmental aneuploidy in human oocytes and preimplantation embryos
Babariya, D., Fragouli, E., Alfarawati, S., Spath, K., & Wells, D. (2017) · Human Reproduction · doi:10.1093/humrep/dex324 · Cited by 142
Self-citationVolozonoka, L., Miskova, A., & Gailite, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23094819 · Cited by 52
Metadata
- Authors
- Wells, D.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-02-15
- Volume
- 27
- Issue
- 4
- Pages
- 1214-1218
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2069652103
- Cited by
- 300
- References
- 0
- FWCI
- 11.82
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation. https://app.cochat.ai/papers/10.1093/nar/27.4.1214
- The paper
- Wells, D. (1999). Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation. Nucleic Acids Research. https://doi.org/10.1093/nar/27.4.1214
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
