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The TIGR Gene Indices: reconstruction and representation of expressed gene sequences

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Quackenbush, J.·2000·Nucleic Acids Research

Abstract

Expressed sequence tags (ESTs) have provided a first glimpse of the collection of transcribed sequences in a variety of organisms. However, a careful analysis of this sequence data can provide significant additional functional, structural and evolutionary information. Our analysis of the public EST sequences, available through the TIGR Gene Indices (TGI; http://www.tigr.org/tdb/tdb.html ), is an attempt to identify the genes represented by that data and to provide additional information regarding those genes. Gene Indices are constructed for selected organisms by first clustering, then assembling EST and annotated gene sequences from GenBank. This process produces a set of unique, high-fidelity virtual transcripts, or tentative consensus (TC) sequences. The TC sequences can be used to provide putative genes with functional annotation, to link the transcripts to mapping and genomic sequence data, and to provide links between orthologous and paralogous genes.

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Metadata

Authors
Quackenbush, J.
Venue
Nucleic Acids Research
Published
2000-01-01
Volume
28
Issue
1
Pages
141-145
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2164429509
Cited by
342
References
1
FWCI
11.27
Type
journal article
Language
en
Open access
green

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Quackenbush, J. (2000). The TIGR Gene Indices: reconstruction and representation of expressed gene sequences. Nucleic Acids Research. https://doi.org/10.1093/nar/28.1.141

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