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New specificity and yield enhancer of polymerase chain reactions
Open accessAbstract
Tetramethylammonium (TMA) chloride, dimethyl sulfoxide and formamide are known to increase, under certain conditions, the specificity and efficiency of the polymerase chain reaction (PCR). We compared the ability of several TMA derivatives and some other reagents to increase the specificity of PCR and to improve the yield of amplification. A novel combination of the enhancer TMA and oxalate as anion is demonstrated to be a powerful enhancer of PCR. Addition of 2 mM TMA oxalate to the PCR mixture decreases the formation of non-specific DNA fragments and increases the yield of specific PCR products.
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“Several tetramethylammonium (TMA) derivatives have been shown to increase the specificity of PCR and improve the yield of amplification products (DiLella and Woo, 1987; Kovarova and Draber, 2000).”
From Short tandem repeat profiling via next-generation sequencing for cell line authentication (2023) · cites this paper as Kovarova and Draber, 2000
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Research works printed but not linked: 15
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Cited by
Short tandem repeat profiling via next-generation sequencing for cell line authentication
Chen, Y.-H., Connelly, J. P., Florian, C., Cui, X., & Pruett-Miller, S. M. (2023) · Disease Models & Mechanisms · doi:10.1242/dmm.050150 · Cited by 10
“Several tetramethylammonium (TMA) derivatives have been shown to increase the specificity of PCR and improve the yield of amplification products (DiLella and Woo, 1987; Kovarova and Draber, 2000).”
Metadata
- Authors
- Kovarova, M., & Draber, P.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-07-01
- Volume
- 28
- Issue
- 13
- Pages
- e70-e70
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC102717
- OpenAlex
- W2150891778
- Cited by
- 77
- References
- 0
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 4
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- PCR, PCR specificity, tetramethylammonium chloride, oxalate, dimethyl sulfoxide, formamide
- MeSH terms
- Quaternary Ammonium Compounds, Animals, Indicators and Reagents, Sensitivity and Specificity, Polymerase Chain Reaction, Thy-1 Antigens, Mice, Rats
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
