A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA
Open accessAbstract
The xeroderma pigmentosum group A protein (XPA) plays a central role in nucleotide excision repair (NER). To identify proteins that bind to XPA, we screened a HeLa cDNA library using the yeast two-hybrid system. Here we report a novel cytoplasmic GTP-binding protein, designated XPA binding protein 1 (XAB1). The deduced amino acid sequence of XAB1 consisted of 374 residues with a molecular weight of 41 kDa and an isoelectric point of 4.65. Sequence analysis revealed that XAB1 has four sequence motifs G1-G4 of the GTP-binding protein family in the N-terminal half. XAB1 also contains an acidic region in the C-terminal portion. Northern blot analysis showed that XAB1 mRNA is expressed ubiquitously, and immunofluorescence analysis revealed that XAB1 is localized mainly in the cytoplasm. Consistent with the GTP-binding motif, purified recombinant XAB1 protein has intrinsic GTPase activity. Using the yeast two-hybrid system, we elucidated that XAB1 binds to the N-terminal region of XPA. The deletion of five amino acids, residues 30-34 of XPA, required for nuclear localization of XPA abolished the interaction with XAB1. These results suggest that XAB1 is a novel cytoplasmic GTPase involved in nuclear localization of XPA.
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XAB1 has been proposed to be the GTPase involved in XPA nuclear import.
“it has been proposed to be the GTPase involved in XPA nuclear import [50]”
From UV-Induced Nuclear Import of XPA Is Mediated by Importin-α4 in An ATR-Dependent Manner (2013) · cites this paper as [50]
In a yeast two-hybrid system, XAB1 was observed to bind XPA.
“In a yeast two-hybrid system XAB1 was observed to bind XPA and it has been proposed to be the GTPase involved in XPA nuclear import [50].”
From UV-Induced Nuclear Import of XPA Is Mediated by Importin-α4 in An ATR-Dependent Manner (2013) · cites this paper as [50]
XAB1 is a GTP-binding protein that was identified as an interacting partner with XPA in a yeast two-hybrid system.
“the protein XAB1 is a GTP-binding protein that was identified as an interacting partner with XPA in a yeast two-hybrid system”
From UV-Induced Nuclear Import of XPA Is Mediated by Importin-α4 in An ATR-Dependent Manner (2013) · cites this paper as [50]
XAB1 was proposed to be the GTPase involved in XPA nuclear import.
“and was proposed to be the GTPase involved in XPA nuclear import [50].”
From UV-Induced Nuclear Import of XPA Is Mediated by Importin-α4 in An ATR-Dependent Manner (2013) · cites this paper as [50]
Following UVB, XPA moves from cytoplasm to nucleus by binding XAB1.
“After UVB irradiation, XPA undergoes a dramatic cytoplasm-to-nucleus translocation via association with cytoplasmic GTPase XPA binding protein 1 (XAB1) (21, 22).”
From Melanocyte-stimulating hormone directly enhances UV-Induced DNA repair in keratinocytes by a xeroderma pigmentosum group A-dependent mechanism (2010) · cites this paper as [21]
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References 33 · Cited by 58
Research works printed but not linked: 4
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- [21]21. Church,G.M. and Gilbert,W. (1984) Proc. Natl Acad. Sci. USA , 81 , 1991-1995.
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- Authors
- Nitta, M.
- Affiliations
- The University of Osaka (JP)
- Corresponding author
- Nitta, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-11-01
- Volume
- 28
- Issue
- 21
- Pages
- 4212-4218
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC113144
- OpenAlex
- W2065942523
- Cited by
- 58
- References
- 33
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 5 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 4 · 2012: 4
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Core Research for Evolutional Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- XPA, nucleotide excision repair, GTPase, protein-protein interaction, nuclear localization
- MeSH terms
- Adenosine Triphosphatases, Amino Acid Sequence, Cytoplasm, DNA-Binding Proteins, Fluorescent Antibody Technique, HeLa Cells, Humans, Male, Molecular Sequence Data, Organ Specificity, Protein Binding, RNA, Messenger, Testis, Xeroderma Pigmentosum, Sequence Alignment, Sequence Deletion, GTP Phosphohydrolases, Two-Hybrid System Techniques, Amino Acid Motifs, Protein Transport, Xeroderma Pigmentosum Group A Protein
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