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Insertion of synthetic copies of human globin genes into bacterial plasmids
Open accessAbstract
Double stranded human globin cDNA was synthesized by use of viral reverse transcriptase from globin mRNA of cord blood of premature infants requiring exchange transfusions. The cDNA was introduced into plasmids and the recombinant DNA plasmids used to transform E. coli X1776. A number of transformants were obtained. Plasmid DNA from selected colonies was isolated and characterized for the type of globin cDNA it contained by three types of procedures: 1) hybridization to previously characterized 3H-labeled alpha,beta and gamma cDNA; 2) analysis of the size and nature of fragments produced by digestion of the plasma DNA by different restriction endonucleases; and 3) by rapid DNA sequence analysis of selected DNA fragments produced by restriction endonuclease digestion. Analysis by these techniques of plasmid DNA from different colonies has definitively identified the presence of human alpha, beta or gamma cDNA sequences in different plasmids.
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Cited by
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,887
Large-scale investigation of the reasons why potentially important genes are ignored
Stoeger, T., Gerlach, M., Morimoto, R. I., & Nunes Amaral, L. A. (2018) · PLOS Biology · doi:10.1371/journal.pbio.2006643 · Cited by 261
Liebhaber, S. A., Cash, F. E., & Ballas, S. K. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66871-1 · Cited by 113
Ross, J., & Sullivan, T. (1985) · Blood · doi:10.1182/blood.v66.5.1149.1149 · Cited by 104
Metadata
- Authors
- Wilson, J. T., Wilson, L. B., deRiel, J. K., Villa-Komaroff, L., Efstratiadis, A., Forget, B. G., & Weissman, S. M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1978
- Volume
- 5
- Issue
- 2
- Pages
- 563-581
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1997554941
- Cited by
- 341
- References
- 0
- FWCI
- 23.97
- Type
- journal article
- Language
- en
- Open access
- green
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- Wilson, J. T., Wilson, L. B., deRiel, J. K., Villa-Komaroff, L., Efstratiadis, A., Forget, B. G., & Weissman, S. M. (1978). Insertion of synthetic copies of human globin genes into bacterial plasmids. Nucleic Acids Research. https://doi.org/10.1093/nar/5.2.563
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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