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DNAase footprinting a simple method for the detection of protein-DNA binding specificity
Open accessAbstract
A method for studying the sequence-specific binding of proteins to DBA is described. The technique is a simple conjoining of the Maxam-Gilbert DNA-sequencing method and the technique of DNAase-protected fragment isolation. Fragments of a 5' end-labelled, double-stranded DNA segment, partially degraded by DNAase in the presence and absence of the binding protein, are visualized by electrophoresis and autoradiography alongside the base-specific reaction products of the Maxam-Gilbert sequencing method. It is then possible to see the protective "footprint" of the binding protein on the DNA sequence. The binding of lac repressor to lac operator is visualized by "footprinting" as an example. Equillibrium estimates indicate that 10-fold sequence-specificity (differential binding constant) could be studied easily using this technique.
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Cited by
Dynan, W. S., & Tjian, R. (1983) · Cell · doi:10.1016/0092-8674(83)90210-6 · Cited by 1,445
Chromatin accessibility profiling by ATAC-seq
Grandi, F. C., Modi, H., Kampman, L., & Corces, M. R. (2022) · Nature Protocols · doi:10.1038/s41596-022-00692-9 · Cited by 557
Global mapping of protein-DNA interactions in vivo by digital genomic footprinting
Hesselberth, J. R., Chen, X., Zhang, Z., Sabo, P. J., Sandstrom, R., Reynolds, A. P., Thurman, R. E., Neph, S., Kuehn, M. S., Noble, W. S., et al. (2009) · Nature Methods · doi:10.1038/nmeth.1313 · Cited by 559
Johnson, P. F., Landschulz, W. H., Graves, B. J., & McKnight, S. L. (1987) · Genes & Development · doi:10.1101/gad.1.2.133 · Cited by 595
Quantitative model for gene regulation by lambda phage repressor.
Ackers, G. K., Johnson, A. D., & Shea, M. A. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.4.1129 · Cited by 590
Metadata
- Authors
- Galas, D. J., & Schmitz, A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1978
- Volume
- 5
- Issue
- 9
- Pages
- 3157-3170
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1989080074
- Cited by
- 2,056
- References
- 0
- FWCI
- 12.87
- Type
- journal article
- Language
- en
- Open access
- green
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- Galas, D. J., & Schmitz, A. (1978). DNAase footprinting a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Research. https://doi.org/10.1093/nar/5.9.3157
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