The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
pUR222, a vector for cloning and rapid chemical sequencing of DNA
Open accessAbstract
A multipurpose plasmid, pUR222, was constructed. It contains six unique cloning sites (PstI, SalI, AccI, HindII, BamHI and EcoRI) in a small region of its lac Z-gene part. Insertion of foreign DNA into the plasmid can be easily detected. Bacteria harbouring recombinant plasmids generally give rise to white colonies, while those containing only vector DNA form blue colonies on indicator plates. Plasmid DNA purified by a rapid method (Birnboim, H.C. and Doly, J. (1979) Nucl. Acids. Res. 7, 1513-1523) can be used for chemical sequencing of the cloned insert DNA. Labeled fragments need not be isolated after cutting with the proper restriction enzymes and are treated directly according to the sequencing protocol of Maxam and Gilbert.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Easy identification of cDNA clones.
Rüther, U., & Müller-Hill, B. (1983) · The EMBO Journal · doi:10.1002/j.1460-2075.1983.tb01659.x · Cited by 573
Self-citationFarinha, M. A., & Kropinski, A. M. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.6.3496-3499.1990 · Cited by 277
Metadata
- Authors
- Ulrich, R., Koenen, M., Otto, K., & Müller-Hill, B.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1981
- Volume
- 9
- Issue
- 16
- Pages
- 4087-4098
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2143301841
- Cited by
- 262
- References
- 1
- FWCI
- 20.47
- Type
- journal article
- Language
- en
- Open access
- green
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- Ulrich, R., Koenen, M., Otto, K., & Müller-Hill, B. (1981). pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/9.16.4087
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