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The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA
Open accessAbstract
Two oligonucleotides 14-bases long were synthesized, one complementary to rabbit beta-globin DNA (R beta G14A) and the other with the same sequence except for a single base change (T for C) (R beta G14B). Hybridization conditions were established such that R beta G14A would hybridize to globin DNA while R beta G14B would not. We also synthesized a mixture of 13-base long oligonucleotides (R beta G13Mix), representing eight of the possible coding sequences for amino acids 15-19 of rabbit beta-globin. One of the eight is complementary to globin DNA. R beta G13Mix was found to hybridize specifically to globin DNA under conditions where oligonucleotides forming single base pair mismatches do not. Furthermore, R beta G13Mix was shown to hybridize specifically to colonies containing a plasmid with a globin DNA insert. These results are discussed with respect to a general procedure for screening recombinant clones for those containing DNA coding for a protein of known amino acid sequence.
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Cited by
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Yang, F., Lum, J. B., McGill, J. R., Moore, C. M., Naylor, S. L., van Bragt, P. H., Baldwin, W. D., & Bowman, B. H. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.9.2752 · Cited by 314
Metadata
- Authors
- Wallace, R. B., Johnson, M. J., Hirose, T., Miyake, T., Kawashima, E. H., & Itakura, K.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1981
- Volume
- 9
- Issue
- 4
- Pages
- 879-894
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2049280274
- Cited by
- 471
- References
- 0
- FWCI
- 17.09
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wallace, R. B., Johnson, M. J., Hirose, T., Miyake, T., Kawashima, E. H., & Itakura, K. (1981). The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/9.4.879
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