Non-canonical Staphylococcus aureus pathogenicity island repression
Open accessPublished version of a preprint
Abstract
Mobile genetic elements control their life cycles by the expression of a master repressor, whose function must be disabled to allow the spread of these elements in nature. Here, we describe an unprecedented repression-derepression mechanism involved in the transfer of Staphylococcus aureus pathogenicity islands (SaPIs). Contrary to the classical phage and SaPI repressors, which are dimers, the SaPI1 repressor StlSaPI1 presents a unique tetrameric conformation never seen before. Importantly, not just one but two tetramers are required for SaPI1 repression, which increases the novelty of the system. To derepress SaPI1, the phage-encoded protein Sri binds to and induces a conformational change in the DNA binding domains of StlSaPI1, preventing the binding of the repressor to its cognate StlSaPI1 sites. Finally, our findings demonstrate that this system is not exclusive to SaPI1 but widespread in nature. Overall, our results characterize a novel repression-induction system involved in the transfer of MGE-encoded virulence factors in nature.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · see citing works
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- Authors
- Miguel-Romero, L., Alqasmi, M., Bacarizo, J., Tan, J. A., Cogdell, R. J., Chen, J., Byron, O., Christie, G. E., Marina, A., & Penadés, J. R.
- Affiliations
- Imperial College London (GB)
- Shaqra University (SA)
- Universidad Cardenal Herrera CEU (ES)
- Virginia Commonwealth University (US)
- University of Glasgow (GB)
- National University of Singapore (SG)
- Centre for Biomedical Network Research on Rare Diseases (ES)
- Corresponding author
- Marina, Alberto
- Penadés, José R
- ORCID
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2022-10-06
- Volume
- 50
- Issue
- 19
- Pages
- 11109-11127
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC9638917
- OpenAlex
- W4302763046
- Cited by
- 9
- References
- 1
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 3
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nucleic Acids Research (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion, cc-by-sa)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
- CEU Repositorio Institucional (Fundación Universitaria San Pablo CEU) (submittedVersion, cc-by-nc-nd)
- Spiral (Imperial College London) (submittedVersion, cc-by)
- Funders
- Medical Research Council (MR/V000772/1, MR/M003876/1, MR/S00940X/1)
- Biotechnology and Biological Sciences Research Council (BB/N002873/1, BB/S003835/1, BB/V002376/1)
- Wellcome Trust (201531/Z/16/Z, ERC-ADG-2014, PID2019-108541GB-I00)
- Spanish Government (MOE2017-T2-2-163, MOE2019-T2-2-162)
- Ministry of Education
- NIH (R01 AI083255)
- NIH IRACDA (K12GM093857)
- Royal Society Wolfson Fellowship
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Staphylococcus aureus, pathogenicity islands, mobile genetic elements, bacteriophages
- MeSH terms
- Staphylococcus Phages, Staphylococcus aureus, Genomic Islands
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