The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Experimental comparison and cross-validation of the Affymetrix and Illumina gene expression analysis platforms
Open accessAbstract
The growth in popularity of RNA expression microarrays has been accompanied by concerns about the reliability of the data especially when comparing between different platforms. Here, we present an evaluation of the reproducibility of microarray results using two platforms, Affymetrix GeneChips and Illumina BeadArrays. The study design is based on a dilution series of two human tissues (blood and placenta), tested in duplicate on each platform. The results of a comparison between the platforms indicate very high agreement, particularly for genes which are predicted to be differentially expressed between the two tissues. Agreement was strongly correlated with the level of expression of a gene. Concordance was also improved when probes on the two platforms could be identified as being likely to target the same set of transcripts of a given gene. These results shed light on the causes or failures of agreement across microarray platforms. The set of probes we found to be most highly reproducible can be used by others to help increase confidence in analyses of other data sets using these platforms.
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Cited by
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From Monocytes to M1/M2 Macrophages: Phenotypical vs. Functional Differentiation
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Regulatory networks define phenotypic classes of human stem cell lines
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Metadata
- Authors
- Barnes, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-10-12
- Volume
- 33
- Issue
- 18
- Pages
- 5914-5923
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2110112697
- Cited by
- 255
- References
- 26
- FWCI
- 8.41
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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