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Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding

Taberlet, P., Coissac, E., Pompanon, F., Gielly, L., Miquel, C., Valentini, A., Vermat, T., Corthier, G., Brochmann, C., Willerslev, E.·2007·Nucleic Acids Research

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Crossref · 10.1093/nar/gkl938

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  • Authentication of metabarcoding results relies on reproducibility across multiple PCR replicates and demonstrating that negative controls are blank or contain different sequence signatures.

    “As with PCR-based aDNA approaches used in the 1990s, the authentication of metabarcoding [G] results relies instead on reproducibility across multiple PCR replicates 128 and demonstrating that negative controls are either blank or contain different sequence signatures than that seen within the samples.”

    From Ancient DNA analysis (2021) · cites this paper as [128]

    The citation follows the claim about reproducibility across multiple PCR replicates. The Materials and Methods section (‘Robustness of the system for biotechnological applications’; page unavailable) describes multiple extraction and PCR blank controls, but does not say that authentication relies on reproducibility across PCR replicates. Searches for replicate amplification and reproducibility found no passage establishing that point. (LLM, conf=0.72)

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Research works printed but not linked: 27

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  7. [7]Vences M. Thomas M. van der Meijden A. Chiari Y. Vieites D. Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians Front. Zool. 2005 2 5 15771783 PMC555853
  8. [8]Hebert P.D.N. Penton E.H. Burns J.M. Janzen D.H. Hallwachs W. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator Proc. Natl Acad. Sci. USA 2004 101 14812 14817 15465915 PMC522015
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  16. [19]Taberlet P. Gielly L. Pautou G. Bouvet J. Universal primers for amplification of three noncoding regions of chloroplast DNA Plant Mol. Biol. 1991 17 1105 1109 1932684
  17. [21]Quandt D. Stech M. Molecular evolution of the trnL (UAA) intron in bryophytes Mol. Phylogenet. Evol. 2005 36 429 443 16005648
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Metadata

Authors
Taberlet, P., Coissac, E., Pompanon, F., Gielly, L., Miquel, C., Valentini, A., Vermat, T., Corthier, G., Brochmann, C., & Willerslev, E.
Venue
Nucleic Acids Research
Published
2007-01-26
Volume
35
Issue
3
Pages
e14-e14
ISSN
  • 0305-1048
  • 1362-4962
Cited by
1,079
References
0
Language
en

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