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Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding
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Authentication of metabarcoding results relies on reproducibility across multiple PCR replicates and demonstrating that negative controls are blank or contain different sequence signatures.
“As with PCR-based aDNA approaches used in the 1990s, the authentication of metabarcoding [G] results relies instead on reproducibility across multiple PCR replicates 128 and demonstrating that negative controls are either blank or contain different sequence signatures than that seen within the samples.”
From Ancient DNA analysis (2021) · cites this paper as [128]
The citation follows the claim about reproducibility across multiple PCR replicates. The Materials and Methods section (‘Robustness of the system for biotechnological applications’; page unavailable) describes multiple extraction and PCR blank controls, but does not say that authentication relies on reproducibility across PCR replicates. Searches for replicate amplification and reproducibility found no passage establishing that point. (LLM, conf=0.72)
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Research works printed but not linked: 27
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“Alternatives, such as trnL, trnH-psbA, and ITS are commonly used (Da Silva et al., 2020; Galimberti et al., 2016; Taberlet et al., 2007; Yang e al., 2016).”
Metadata
- Authors
- Taberlet, P., Coissac, E., Pompanon, F., Gielly, L., Miquel, C., Valentini, A., Vermat, T., Corthier, G., Brochmann, C., & Willerslev, E.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-01-26
- Volume
- 35
- Issue
- 3
- Pages
- e14-e14
- ISSN
- 0305-1048
- 1362-4962
- PMC
- PMC1807943
- Cited by
- 1,079
- References
- 0
- Language
- en
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