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Cited by 68MicroRNA in disease regulationCell Adhesion Molecules ResearchDevelopmental Biology and Gene Regulation

MicroRNA-338-3p and microRNA-451 contribute to the formation of basolateral polarity in epithelial cells

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Tsuchiya, Soken, Oku, Masahito, Imanaka, Yukako, Kunimoto, Ryo, Okuno, Yasushi, Terasawa, Kazuya, Sato, Fumiaki, Tsujimoto, Gozoh +1 more·2009·Nucleic Acids Research
DOI 10.1093/nar/gkp255PMC PMC2699527
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Abstract

MicroRNAs are small noncoding RNA species, some of which are playing important roles in cell differentiation. However, the level of participations of microRNAs in epithelial cell differentiation is largely unknown. Here, utilizing an epithelial differentiation model with T84 cells, we demonstrate that miR-338-3p and miR-451 contribute to the formation of epithelial basolateral polarity by facilitating translocalization of beta1 integrin to the basolateral membrane. Among 250 microRNAs screened in this study, the expression levels of four microRNAs (miR-33a, 210, 338-3p and 451) were significantly elevated in the differentiated stage of T84 cells, when epithelial cell polarity was established. To investigate the involvement of these microRNAs in terms of epithelial cell polarity, we executed loss-of- and gain-of-function analyses of these microRNAs. The blockade of endogenous miR-338-3p or miR-451 via each microRNA-specific antisense oligonucleotides inhibited the translocalization of beta1 integrin to the basolateral membrane, whereas inhibition of miR-210 or miR-33a had no effect on it. On the other hand, simultaneous transfection of synthetic miR-338-3p and miR-451 accelerated the translocalization of beta1 integrin to the basolateral membrane, although the introduction of individual synthetic microRNAs exhibited no effect. Therefore, we concluded that both miR-338-3p and miR-451 are necessary for the development of epithelial cell polarity.

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  • Cellular miR-33 levels are dependent on culture conditions and exogenous lipid supply.

    “It has been previously noted that cellular miR-33 levels are dependent on culture conditions and exogenous lipid supply (36).”

    From Expression of miR-33 from an SREBP2 Intron Inhibits Cholesterol Export and Fatty Acid Oxidation* (2010) · cites this paper as [36]

    The Results (Screening of miRNAs related with differentiation; page unavailable) report that miR-33a increased in T84 cells between culture days 0 and 7, supporting a limited link between its level and culture conditions. The paper does not report that exogenous lipid supply affects miR-33 levels; its cell-culture methods describe serum-containing medium, not a lipid-supply comparison. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • miR-338-3p regulates morphological polarity in other cell types by facilitating the activity of β-integrin.

    “Others have shown miR-338-3p regulates morphological polarity in other cell types by facilitating the activity of β-integrin [26].”

    From MiR-338-3p regulates neuronal maturation and suppresses glioblastoma proliferation. (2017) · cites this paper as [26]

    The Results section (Roles of miRNAs in basolateral polarity; page unavailable) shows that miR-338-3p contributes to basolateral polarity in T84 epithelial cells by promoting β1-integrin localization to the basolateral membrane. It does not show increased β-integrin activity, and the effect required miR-451 as well; miR-338-3p alone was insufficient. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Authors
Tsuchiya, S., Oku, M., Imanaka, Y., Kunimoto, R., Okuno, Y., Terasawa, K., Sato, F., Tsujimoto, G., & Shimizu, K.
Affiliations
Kyoto University (JP)
Corresponding author
Shimizu, Kazuharu
ORCID
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2009-04-22
Volume
37
Issue
11
Pages
3821-3827
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/gkp255
PMC
PMC2699527
OpenAlex
W2082496751
Cited by
68
References
2
Citations by year
2025: 1 · 2023: 1 · 2021: 7 · 2020: 5 · 2019: 5 · 2018: 8 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 1 · 2013: 7 · 2012: 7
FWCI
2.80
Type
journal article
Language
en
Open access
Licence
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • Johns Hopkins University
  • National Institute of Biomedical Innovation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
microRNA-451, epithelial polarity, beta1 integrin, epithelial differentiation, T84 cells, miR-33a, miR-210
MeSH terms
Cell Differentiation, Epithelial Cells, Gene Expression Regulation, Humans, Cell Polarity, MicroRNAs, Cell Line, Tumor

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