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Codon reassignment in the Escherichia coli genetic code
Open accessAbstract
Most organisms, from Escherichia coli to humans, use the 'universal' genetic code, which have been unchanged or 'frozen' for billions of years. It has been argued that codon reassignment causes mistranslation of genetic information, and must be lethal. In this study, we successfully reassigned the UAG triplet from a stop to a sense codon in the E. coli genome, by eliminating the UAG-recognizing release factor, an essential cellular component, from the bacterium. Only a few genetic modifications of E. coli were needed to circumvent the lethality of codon reassignment; erasing all UAG triplets from the genome was unnecessary. Thus, UAG was assigned unambiguously to a natural or non-natural amino acid, according to the specificity of the UAG-decoding tRNA. The result reveals the unexpected flexibility of the genetic code.
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Cites
Rewiring the keyboard: evolvability of the genetic code
Knight, R. D., Freeland, S. J., & Landweber, L. F. (2001) · Nature Reviews Genetics · doi:10.1038/35047500 · Cited by 435
Cited by
Expanding and Reprogramming the Genetic Code of Cells and Animals
Chin, J. W. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035737 · Cited by 476
Expanding the Genetic Code of Escherichia coli with Phosphoserine
Park, H.-S., Hohn, M. J., Umehara, T., Guo, L.-T., Osborne, E. M., Benner, J., Noren, C. J., Rinehart, J., & Söll, D. (2011) · Science · doi:10.1126/science.1207203 · Cited by 377
Schmied, W. H., Elsässer, S. J., Uttamapinant, C., & Chin, J. W. (2014) · Journal of the American Chemical Society · doi:10.1021/ja5069728 · Cited by 270
Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology
Ling, J., O'Donoghue, P., & Söll, D. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3568 · Cited by 135
Metadata
- Authors
- Mukai, T., Hayashi, A., Iraha, F., Sato, A., Ohtake, K., Yokoyama, S., & Sakamoto, K.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-08-11
- Volume
- 38
- Issue
- 22
- Pages
- 8188-8195
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1969330612
- Cited by
- 200
- References
- 1
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- gold
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- Mukai, T., Hayashi, A., Iraha, F., Sato, A., Ohtake, K., Yokoyama, S., & Sakamoto, K. (2010). Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Research. https://doi.org/10.1093/nar/gkq707
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