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Cited by 50MicroRNA in disease regulationRNA Research and SplicingPlant Molecular Biology Research

Uracils at nucleotide position 9–11 are required for the rapid turnover of miR-29 family

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Zhang, Zhuo, Zou, Jun, Wang, Guo-Kun, Zhang, Jun-Tao, Huang, Shuang, Qin, Yong-Wen, Jing, Qing·2011·Nucleic Acids Research
DOI 10.1093/nar/gkr020PMC PMC3105410
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Abstract

MicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulse-chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9-11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.

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  • miR-29a is the most abundantly expressed and stable among the miR-29 family.

    “miR-29a, which is the most abundantly expressed and stable among the family [43]”

    From miRNA family miR-29 inhibits PINK1-PRKN signaling via ATG9A (2026) · cites this paper as [43]

    The Results (Figure 3B and the section on rapid turnover) show that miR-29a is more stable than miR-29b and miR-29c in the authors’ HeLa-cell assay. The paper does not establish that miR-29a is the most abundantly expressed family member; its abundance measurements concern transfected miRNA mimickers rather than a comparison of endogenous family-member expression. No page numbers are available in our copy. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • miR-29b and miR-29c share a tri-uracil element at nucleotide positions 9-11 that causes their rapid decay.

    “miR-29b and miR-29c share a tri-uracil element at nucleotide positions 9–11 that causes their rapid decay [43].”

    From miRNA family miR-29 inhibits PINK1-PRKN signaling via ATG9A (2026) · cites this paper as [43]

    In Results (“Uracils at nucleotide position 9–11 of miR-29b are required for its rapid decay”; page unavailable), replacing those uracils stabilizes miR-29b, supporting their role in its rapid decay. Results (“A subset of miRNAs with uracils at nucleotide positions 9–11 possesses rapid turnover”; page unavailable) reports relatively rapid turnover for miR-29c, but does not establish that the element causes miR-29c’s decay. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

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Authors
Zhang, Z., Zou, J., Wang, G.-K., Zhang, J.-T., Huang, S., Qin, Y.-W., & Jing, Q.
Affiliations
Shanghai Jiao Tong University (CN)
Corresponding author
  • Qin, Yong-Wen
  • Jing, Qing
ORCID
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2011-02-01
Volume
39
Issue
10
Pages
4387-4395
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/gkr020
PMC
PMC3105410
OpenAlex
W2137544017
Cited by
50
References
1
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2022: 4 · 2021: 5 · 2020: 2 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 5 · 2014: 6 · 2013: 3 · 2012: 7
FWCI
1.85
Type
journal article
Language
en
Open access
Licence
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (30770457)
  • Chinese Academy of Sciences (KSCX2-YW-R)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
microRNA degradation, miR-29b, RNA duplex, sequence context, miR-29c, bantam microRNA, microRNA biogenesis
MeSH terms
HeLa Cells, Humans, RNA, Double-Stranded, Uracil, RNA Stability, MicroRNAs

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