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Cited by 53RNA and protein synthesis mechanismsAnimal Virus Infections StudiesViral Infectious Diseases and Gene Expression in Insects

The IRES5′UTR of the dicistrovirus cricket paralysis virus is a type III IRES containing an essential pseudoknot structure

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Gross, Lauriane, Vicens, Quentin, Einhorn, Evelyne, Noireterre, Audrey, Schaeffer, Laure, Kuhn, Lauriane, Imler, Jean-Luc, Eriani, Gilbert +2 more·2017·Nucleic Acids Research
DOI 10.1093/nar/gkx622PMC PMC5587806
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Abstract

Cricket paralysis virus (CrPV) is a dicistrovirus. Its positive-sense single-stranded RNA genome contains two internal ribosomal entry sites (IRESs). The 5' untranslated region (5'UTR) IRES5'UTR mediates translation of non-structural proteins encoded by ORF1 whereas the well-known intergenic region (IGR) IRESIGR is required for translation of structural proteins from open reading frame 2 in the late phase of infection. Concerted action of both IRES is essential for host translation shut-off and viral translation. IRESIGR has been extensively studied, in contrast the IRES5'UTR remains largely unexplored. Here, we define the minimal IRES element required for efficient translation initiation in drosophila S2 cell-free extracts. We show that IRES5'UTR promotes direct recruitment of the ribosome on the cognate viral AUG start codon without any scanning step, using a Hepatitis-C virus-related translation initiation mechanism. Mass spectrometry analysis revealed that IRES5'UTR recruits eukaryotic initiation factor 3, confirming that it belongs to type III class of IRES elements. Using Selective 2'-hydroxyl acylation analyzed by primer extension and DMS probing, we established a secondary structure model of 5'UTR and of the minimal IRES5'UTR. The IRES5'UTR contains a pseudoknot structure that is essential for proper folding and ribosome recruitment. Overall, our results pave the way for studies addressing the synergy and interplay between the two IRES from CrPV.

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  • The IRES from Cricket paralysis virus (CrPV) is able to directly recruit the 60S subunit.

    “the IRES from Cricket paralysis virus (CrPV) that is able to directly recruit the 60S subunit.29”

    From Evaluation of synthetic mRNA with selected UTR sequences and alternative poly(A) tail, in vitro and in vivo (2025) · cites this paper as [29]

    The Introduction (page unavailable) says type III IRESs bind directly to the 40S ribosomal subunit, and the paper identifies the CrPV 5′UTR IRES as type III. It does not say that this IRES directly recruits the 60S subunit; the specified subunit is contrary to the claim. (LLM, conf=0.88)

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Research works printed but not linked: 53

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Authors
Gross, L., Vicens, Q., Einhorn, E., Noireterre, A., Schaeffer, L., Kuhn, L., Imler, J.-L., Eriani, G., Meignin, C., & Martin, F.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Martin, Franck
ORCID
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2017-07-18
Volume
45
Issue
15
Pages
8993-9004
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/gkx622
PMC
PMC5587806
OpenAlex
W2734396391
Cited by
53
References
0
Citations by year
2025: 3 · 2024: 8 · 2023: 2 · 2022: 9 · 2021: 11 · 2020: 6 · 2019: 7 · 2018: 5 · 2017: 1 · 2016: 1
FWCI
2.07
Type
journal article
Language
en
Open access
Licence
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
Funders
Agence Nationale de la Recherche (ANR-10-LABX-0036)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords
cricket paralysis virus, dicistrovirus, pseudoknot, ribosome recruitment, translation initiation, 5' untranslated region, RNA secondary structure, Drosophila S2 cells
MeSH terms
Internal Ribosome Entry Sites, Animals, Base Sequence, Cell Line, Cell-Free System, Drosophila melanogaster, Gryllidae, Nucleic Acid Conformation, Ribosomes, RNA, Viral, Protein Biosynthesis, Viral Proteins, Open Reading Frames, 5' Untranslated Regions, Host-Pathogen Interactions, Dicistroviridae

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