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Cited by 41Genetic and Kidney Cyst DiseasesATP Synthase and ATPases ResearchCystic Fibrosis Research Advances

The DOI matches its Crossref record.

Effects of N-Ethylmaleimide on Dynein Adenosinetriphosphatase Activity and Its Recombining Ability with Outer Fibers

Takashi Shimizu, Ichirô KIMURA·1974·The Journal of Biochemistry
DOI 10.1093/oxfordjournals.jbchem.a130640
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Abstract

The effects of N-ethylmaleimide (NEM) on ciliary ATPase (dyneins) [EC 3.6.1.3] of Tetrahymena pyriformis were investigated. Like muscle myosin ATPase, 30 S dynein ATPase was activated biphasically by NEM, while 14 S dynein ATPase was simply inhibited. The maximal activation of 30 S dynein ATPase was obtained by preincubation with NEM at a concentration of 50 μM (0.23 μmole NEM/mg protein) and the magnitude of activation was 2.5- to 3-fold. The changes in the characteristics of 30 S dynein ATPase caused by modification were studied in comparison with those of myosin ATPase. It was confirmed that 30 S dynein ATPase was a myosin-like enzyme, though a few differences were found. From recombination tests of modified 30 S dynein with outer fibers, it was shown that at least three types of -SH groups are present in 30 S dynein molecules; -SH groups responsible for recombination with outer fibers, for enhancement of the enzyme activity by their blockage, and for catalytic activity.

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Metadata

Authors
Shimizu, T., & KIMURA, I.
Affiliations
The University of Tokyo (JP)
ORCID
Venue
The Journal of Biochemistry
Publisher
Oxford University Press (OUP)
Published
1974-11
Volume
76
Issue
5
Pages
1001-8
ISSN
  • 0021-924X (ISSN-L)
  • 1756-2651
DOI
10.1093/oxfordjournals.jbchem.a130640
OpenAlex
W884304900
Cited by
41
References
0
Citations by year
2023: 1 · 2017: 1 · 2014: 1 · 2012: 1
FWCI
2.03
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
Keywords
N-ethylmaleimide, dynein ATPase, Tetrahymena pyriformis, enzyme activation, sulfhydryl groups, catalytic activity
MeSH terms
Adenosine Triphosphatases, Cilia, Enzyme Activation, Ethylmaleimide, Hydrogen-Ion Concentration, Potassium Chloride, Temperature, Tetrahymena, Trichloroacetic Acid

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