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Purification of Ascorbate Peroxidase in Spinach Chloroplasts; Its Inactivation in Ascorbate-Depleted Medium and Reactivation by Monodehydroascorbate Radical
Abstract
Ascorbate specific peroxidase in chloroplasts was purified from spinach leaves. Spinach chloroplast peroxidase was a monomer with a molecular weight of about 30,000 and showed an absorption spectrum similar to a hemoprotein. The enzyme lost its activity within a minute in the absence of ascorbate under aerobic conditions. In addition to ascorbate, 20% sorbitol was necessary to stabilize the enzyme. The inactivation of the enzyme in the ascorbate-depleted medium was protected by other electron donors, pyrogallol, guaiacol and pyrocatechol, whose oxidation rates were very low compared with that of ascorbate. The inactivated enzyme recovered its activity with monodehydroascorbate radicals generated by the ascorbate-ascorbate oxidase system. A mechanism of inactivation and reactivation of ascorbate peroxidase is proposed.
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Cited by
Polle, A. (2001) · Plant Physiology · doi:10.1104/pp.126.1.445 · Cited by 420
Changes in Salicylic Acid and Antioxidants during Induced Thermotolerance in Mustard Seedlings
Dat, J. F., Foyer, C. H., & Scott, I. M. (1998) · Plant Physiology · doi:10.1104/pp.118.4.1455 · Cited by 406
Farooq, M., Wahid, A., & Lee, D.-J. (2009) · Acta Physiologiae Plantarum · doi:10.1007/s11738-009-0307-2 · Cited by 295
Zhang, Z., Yang, D., Yang, B., Gao, Z., Li, M., Jiang, Y., & Hu, M. (2013) · Scientia Horticulturae · doi:10.1016/j.scienta.2013.05.023 · Cited by 84
Nurzhanova, A. A., Pidlisnyuk, V., Berzhanova, R., Nurmagambetova, A. S., Terletskaya, N., Omirbekova, N., Berkinbayev, G., & Mamirova, A. (2023) · Environmental Science and Pollution Research · doi:10.1007/s11356-023-29031-5 · Cited by 18
Metadata
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1987-01
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W1872812400
- Cited by
- 801
- References
- 0
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Purification of Ascorbate Peroxidase in Spinach Chloroplasts; Its Inactivation in Ascorbate-Depleted Medium and Reactivation by Monodehydroascorbate Radical. https://app.cochat.ai/papers/10.1093/oxfordjournals.pcp.a077268
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- Purification of Ascorbate Peroxidase in Spinach Chloroplasts; Its Inactivation in Ascorbate-Depleted Medium and Reactivation by Monodehydroascorbate Radical. (1987). Plant and Cell Physiology. https://doi.org/10.1093/oxfordjournals.pcp.a077268
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