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A Null Mutation in a bZIP Factor Confers ABA-Insensitivity in Arabidopsis thaliana

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Lopez-Molina, L., Chua, N.-H.·2000·Plant and Cell Physiology

Abstract

We have used a modification of the classical ABA-insensitive screen (Koornneef et al. 1984) to isolate novel mutations in the ABA signal transduction pathway of Arabidopsis thaliana. In our screen, mutants were recovered on the basis of their growth-insensitivity to ABA (GIA) rather than germination-insensitivity. Here we present the isolation of the gia1 mutant as well as the identification of the gia1 gene by positional cloning and complementation studies. GIA1 is predicted to code for a bZIP transcription factor with high homology to previously characterized plant bZIP transcription factors (DPBF1, ABFs and TRAB1) known for their ability to bind ABA-responsive DNA elements. Our results provide in vivo evidence that a bZIP factor may indeed be involved in ABA signaling. Since GIA1 turned out to be identical to ABI5, we designated GIA1 as ABI5 in the present paper.

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Metadata

Authors
Lopez-Molina, L., & Chua, N.-H.
Venue
Plant and Cell Physiology
Published
2000-05-01
Volume
41
Issue
5
Pages
541-547
ISSN
  • 0032-0781 (ISSN-L)
  • 1471-9053
OpenAlex
W2112259299
Cited by
297
References
0
FWCI
19.07
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: A Null Mutation in a bZIP Factor Confers ABA-Insensitivity in Arabidopsis thaliana. https://app.cochat.ai/papers/10.1093/pcp/41.5.541
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Lopez-Molina, L., & Chua, N.-H. (2000). A Null Mutation in a bZIP Factor Confers ABA-Insensitivity in Arabidopsis thaliana. Plant and Cell Physiology. https://doi.org/10.1093/pcp/41.5.541

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