Plant-on-chip: Core morphogenesis processes in the tiny plant Wolffia australiana
Open accessPublished version of a preprint
Abstract
A plant can be thought of as a colony comprising numerous growth buds, each developing to its own rhythm. Such lack of synchrony impedes efforts to describe core principles of plant morphogenesis, dissect the underlying mechanisms, and identify regulators. Here, we use the minimalist known angiosperm to overcome this challenge and provide a model system for plant morphogenesis. We present a detailed morphological description of the monocot Wolffia australiana, as well as high-quality genome information. Further, we developed the plant-on-chip culture system and demonstrate the application of advanced technologies such as single-nucleus RNA-sequencing, protein structure prediction, and gene editing. We provide proof-of-concept examples that illustrate how W. australiana can decipher the core regulatory mechanisms of plant morphogenesis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · see citing works
PEP1 regulates perennial flowering in Arabis alpina
Wang, R., Farrona, S., Vincent, C., Joecker, A., Schoof, H., Turck, F., Alonso-Blanco, C., Coupland, G., & Albani, M. C. (2009) · Nature · doi:10.1038/nature07988 · Cited by 376 · see citing works
Profiling cellular diversity in sponges informs animal cell type and nervous system evolution
Musser, J. M., Schippers, K. J., Nickel, M., Mizzon, G., Kohn, A. B., Pape, C., Ronchi, P., Papadopoulos, N., Tarashansky, A. J., Hammel, J. U., et al. (2021) · Science · doi:10.1126/science.abj2949 · Cited by 242 · see citing works
Flagged first, then by citations
- Authors
- Li, F., Yang, J.-J., Sun, Z.-Y., Wang, L., Qi, L.-Y., A, S., Liu, Y.-Q., Zhang, H.-M., Dang, L.-F., Wang, S.-J., Luo, C.-X., Nian, W.-F., O’Conner, S., Ju, L.-Z., Quan, W.-P., Li, X.-K., Wang, C., Wang, D.-P., You, H.-L., Cheng, Z.-K., Yan, J., Tang, F.-C., Yang, D.-C., Xia, C.-W., Gao, G., Wang, Y., Zhang, B.-C., Zhou, Y.-H., Guo, X., Xiang, S.-H., Liu, H., Peng, T.-B., Su, X.-D., Chen, Y., Ouyang, Q., Wang, D.-H., Zhang, D.-M., Xu, Z.-H., Hou, H.-W., Bai, S.-N., & Li, L.
- Affiliations
- Peking University (CN)
- Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan 430072 , China
- GrandOmics Biosciences Ltd. , Wuhan 430076 , China
- Mississippi State University (US)
- Renmin University of China (CN)
- The High School Affiliated to Renmin University of China , Beijing 100080 , China
- College of Life Sciences, Peking University , Beijing 100871 , China
- Center of Quantitative Biology, Peking University , Beijing 100871 , China
- Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University , Yangzhou 225009 , China
- Yangzhou University (CN)
- BGI Genomics (CN)
- State Key Laboratory of Protein & Plant Gene Research, Peking University , Beijing 100871 , China
- Centre National de la Recherche Scientifique (FR)
- Institute of Botany, Chinese Academy of Sciences , Beijing 100093 , China
- Chinese Academy of Sciences (CN)
- Corresponding author
- Hou, Hong-Wei
- Bai, Shu-Nong
- Li, Ling
- ORCID
- Venue
- PNAS Nexus
- Publisher
- Oxford University Press (OUP)
- Published
- 2023-04-19
- Volume
- 2
- Issue
- 5
- Pages
- pgad141-pgad141
- ISSN
- 2752-6542 (ISSN-L)
- PMC
- PMC10169700
- OpenAlex
- W4366407479
- Cited by
- 15
- References
- 3
- Citations by year
- 2026: 8 · 2025: 4 · 2024: 2 · 2023: 1
- FWCI
- 2.36
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PNAS Nexus (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Greater South Information System
- Funders
- U.S. National Science Foundation (2238942)
- Mississippi State University
- International Partnership Program of Chinese Academy of Sciences (152342KYSB20200021)
- National Natural Science Foundation of China (32001107, 10721403)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- plant morphogenesis, genome assembly, single-nucleus RNA sequencing, protein structure prediction, genome editing
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
