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A synthetic IgG-binding domain based on staphylococcal protein A
Abstract
A synthetic IgG-binding domain based on staphylococcal protein A was designed with the aid of sequence comparisons and computer graphic analysis. A strategy, utilizing non-palindromic restriction sites, was used to overcome the difficulties of introducing site-specific changes into the repetitive gene. A single mutagenized gene fragment was polymerized to different multiplicities, and the different gene products were expressed in Escherichia coli. Using this scheme, protein A-like proteins composed of different numbers of IgG-binding domains were produced. These domains were changed to lack asparagine--glycine dipeptide sequences as well as methionine residues and are thus, in contrast to native protein A, resistant to treatment with hydroxylamine and cyanogen bromide.
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Cited by
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Metadata
- Authors
- Nilsson, B., Moks, T., Jansson, B., Abrahmsén, L., Elmblad, A., Holmgren, E., Henrichson, C., Jones, T. A., & Uhlén, M.
- Venue
- "Protein Engineering, Design and Selection"
- Publisher
- Oxford University Press (OUP)
- Published
- 1987
- Volume
- 1
- Issue
- 2
- Pages
- 107-113
- ISSN
- 0269-2139 (ISSN-L)
- 1741-0126
- 1741-0134
- OpenAlex
- W1993870692
- Cited by
- 759
- References
- 0
- FWCI
- 8.83
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A synthetic IgG-binding domain based on staphylococcal protein A. https://app.cochat.ai/papers/10.1093/protein/1.2.107
- The paper
- Nilsson, B., Moks, T., Jansson, B., Abrahmsén, L., Elmblad, A., Holmgren, E., Henrichson, C., Jones, T. A., & Uhlén, M. (1987). A synthetic IgG-binding domain based on staphylococcal protein A. "Protein Engineering, Design and Selection". https://doi.org/10.1093/protein/1.2.107
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
